生物来源
mouse
质量水平
抗体形式
purified antibody
抗体产品类型
primary antibodies
克隆
TAU-5, monoclonal
表单
liquid
不包含
preservative
种属反应性
mouse, human, sheep, rat
制造商/商品名称
Calbiochem®
储存条件
OK to freeze
avoid repeated freeze/thaw cycles
dilution
(Frozen Sections (1-2 µg/mL)
Immunoblotting (1 µg/mL)
Immunoprecipitation (10 µg per 200-500 µg cell lysate)
Paraffin Sections (1-2 µg/mL, heat pre-treatment required))
同位素/亚型
IgG1
运输
wet ice
储存温度
−70°C
靶向翻译后修饰
unmodified
基因信息
human ... MAPT(4137)
mouse ... Mapt(17762)
rat ... Mapt(29477)
一般描述
蛋白G纯化的小鼠单克隆抗体。识别约45-68 kDa磷酸化和非磷酸化形式的tau。
识别~45-68 kDa Tau蛋白。
该小鼠抗Tau mAb(TAU-5)经过验证，可用于冷冻切片、免疫印迹、免疫沉淀、石蜡切片以检测Tau。
免疫原
牛
纯化的牛微管相关蛋白
表位：中心区域内
应用
冷冻切片（1-2 µg/ml)
免疫印迹（1 µg/ml）
免疫沉淀（每200-500 µg细胞裂解液 10 µg）
石蜡切片（1-2 µg/ml，需要热预处理）
免疫印迹（1 µg/ml）
免疫沉淀（每200-500 µg细胞裂解液 10 µg）
石蜡切片（1-2 µg/ml，需要热预处理）
包装
特定浓度批号请参考小瓶的标签。
外形
在PBS中。
制备说明
初次解冻后，分装冻存（-70°C）。如果浓度为<50 µg/ml，请勿贮藏没有载体蛋白的稀释抗体。
其他说明
Papasozomenos, S.C. and Shanavas, A., 2002.Proc.Natl.Acad.Sci. USA99, 1140.
Rapoport, M., eta al.2002.Proc.Natl.Acad.Sci. USA99, 6364.
Rapoport, M., eta al.2002.Proc.Natl.Acad.Sci. USA99, 6364.
当用于福尔马林/石蜡包埋切片时，在10 mM枸橼酸盐缓冲液（pH 6.0）中煮沸组织切片10-20分钟，然后在抗体培养前在室温下冷却20分钟，从而增强染色。Tau mRNA的交替剪接和差异磷酸化促成tau的异质性。不与微管蛋白的其他MAPS发生交叉反应。识别天然和磷酸化形式的tau。合适的工作稀释度由与测定条件相关的变量决定。
法律信息
CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany
免责声明
毒性：标准处理（A）
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储存分类代码
12 - Non Combustible Liquids
WGK
WGK 1
闪点(°F)
Not applicable
闪点(°C)
Not applicable
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