产品名称
Tau小鼠单克隆抗体(TAU-5), liquid, clone TAU-5, Calbiochem®
biological source
mouse
antibody form
purified antibody
antibody product type
primary antibodies
clone
TAU-5, monoclonal
form
liquid
does not contain
preservative
species reactivity
mouse, human, sheep, rat
manufacturer/tradename
Calbiochem®
storage condition
OK to freeze
avoid repeated freeze/thaw cycles
dilution
(Frozen Sections (1-2 µg/mL)
Immunoblotting (1 µg/mL)
Immunoprecipitation (10 µg per 200-500 µg cell lysate)
Paraffin Sections (1-2 µg/mL, heat pre-treatment required))
isotype
IgG1
shipped in
wet ice
storage temp.
−70°C
target post-translational modification
unmodified
Quality Level
Gene Information
human ... MAPT(4137)
mouse ... Mapt(17762)
rat ... Mapt(29477)
Application
冷冻切片（1-2 µg/ml)
免疫印迹（1 µg/ml）
免疫沉淀（每200-500 µg细胞裂解液 10 µg）
石蜡切片（1-2 µg/ml，需要热预处理）
免疫印迹（1 µg/ml）
免疫沉淀（每200-500 µg细胞裂解液 10 µg）
石蜡切片（1-2 µg/ml，需要热预处理）
Disclaimer
毒性：标准处理（A）
General description
蛋白G纯化的小鼠单克隆抗体。识别约45-68 kDa磷酸化和非磷酸化形式的tau。
识别~45-68 kDa Tau蛋白。
该小鼠抗Tau mAb(TAU-5)经过验证，可用于冷冻切片、免疫印迹、免疫沉淀、石蜡切片以检测Tau。
Immunogen
牛
纯化的牛微管相关蛋白
表位：中心区域内
Other Notes
Papasozomenos, S.C. and Shanavas, A., 2002.Proc.Natl.Acad.Sci. USA99, 1140.
Rapoport, M., eta al.2002.Proc.Natl.Acad.Sci. USA99, 6364.
Rapoport, M., eta al.2002.Proc.Natl.Acad.Sci. USA99, 6364.
当用于福尔马林/石蜡包埋切片时，在10 mM枸橼酸盐缓冲液（pH 6.0）中煮沸组织切片10-20分钟，然后在抗体培养前在室温下冷却20分钟，从而增强染色。Tau mRNA的交替剪接和差异磷酸化促成tau的异质性。不与微管蛋白的其他MAPS发生交叉反应。识别天然和磷酸化形式的tau。合适的工作稀释度由与测定条件相关的变量决定。
Packaging
特定浓度批号请参考小瓶的标签。
Physical form
在PBS中。
Preparation Note
初次解冻后，分装冻存（-70°C）。如果浓度为<50 µg/ml，请勿贮藏没有载体蛋白的稀释抗体。
Legal Information
CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany
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存储类别
12 - Non Combustible Liquids
wgk
WGK 1
flash_point_f
Not applicable
flash_point_c
Not applicable
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The analysis of global and comparative genomics between different diseases allows us to understand the key biological processes that explain the etiology of these pathologies. We have used this type of approach to evaluate the expression of several neurodegeneration-related genes
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