TGF-α, Human, Recombinant, E. coli

Recombinant, human transforming growth factor-α expressed in E. coli. Plays an important role in cell-cell adhesion and cell growth and differentiation. Reported to play a role in the development of hormonal insensitivity in estrogen-receptor-positive breast cancers.

ED₅₀= 0.1-0.4 ng/ml as measured by the ability to stimulate ³H-thymidine incorporation in the mouse fibroblast cell line Balb/3T3

Lyophilized from 30% acetonitrile, 0.1% TFA.

Following reconstitution, aliquot and freeze (-70°C) for long-term storage or refrigerate (4°C) for short-term storage. Avoid freeze/thaw cycles of solutions. Stock solutions are stable for up to 1 month at 4°C or for up to 3 months at -70°C.

Reconstitute to a concentration of ≥10 µg/ml with sterile 10 mM acetic acid containing ≥0.1% HSA or BSA.

Nicholson, R.I., et al. 1994. Cancer Res. 54, 1684.
Anklesaria, P., et al. 1990. Proc. Natl. Acad. Sci. USA87, 3289.
Massague, J. 1990. J. Biol. Chem.265, 21393.
Watanabe, S., et al. 1987. Proc. Natl. Acad. Sci. USA84, 1258.
Schreiber, A.B., et al. 1986. Science232, 1250.
Tam, J.P. 1985. Science229, 673.
Derynck, R., et al. 1984. Cell38, 327.

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Toxicity: Standard Handling (A)