biological source
rabbit
Quality Level
antibody product type
primary antibodies
clone
polyclonal
form
liquid
contains
≤0.1% sodium azide as preservative
species reactivity
rat, human, mouse
manufacturer/tradename
Calbiochem®
storage condition
do not freeze
isotype
IgG
shipped in
wet ice
storage temp.
2-8°C
General description
Protein A purified rabbit polyclonal antibody. Recognizes the ~77-85 kDa transglutaminase II protein.
Recognizes the ~77-85 kDa transglutaminase II protein.
This Anti-Transglutaminase II Rabbit pAb is validated for use in Frozen Sections, Immunoblotting, Immunoprecipitation, Paraffin Sections for the detection of Transglutaminase II.
Immunogen
Human
full-length, recombinant, human cellular/tissue transglutaminase II
Application
Frozen Sections (5-10 µg/ml)
Immunoblotting (5 µg/ml)
Immunoprecipitation (10 µg/mg protein from lysate)
Paraffin Sections (5-10 µg/ml, heat pre-treatment required)
Immunoblotting (5 µg/ml)
Immunoprecipitation (10 µg/mg protein from lysate)
Paraffin Sections (5-10 µg/ml, heat pre-treatment required)
Packaging
Please refer to vial label for lot-specific concentration.
Physical form
In 10 mM PBS, 0.2% BSA, pH 7.4
Other Notes
De Laurenzi, V., and Melino, G. 2001. Mol. Cell. Biol.21, 148.
Nanda, N., et al. 2001. J. Biol. Chem.276, 20673.
Kim, S.Y., and Grant, P.l. 1999. J. Biol. Chem.274, 30715.
Nanda, N., et al. 2001. J. Biol. Chem.276, 20673.
Kim, S.Y., and Grant, P.l. 1999. J. Biol. Chem.274, 30715.
Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at room temperature for 20 min. Does not cross-react with Factor XIIIa, keratinocyte TGase I, fibrinogen, fibronectin, collagen, or actin. Variables associated with assay conditions will dictate the optimal working dilution.
Legal Information
CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
Toxicity: Standard Handling (A)
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存储类别
10-13 - German Storage Class 10 to 13
法规信息
监管及禁止进口产品
此项目有
Maria J Serrano et al.
Journal of cellular physiology, 230(12), 2961-2972 (2015-04-22)
The mammalian secondary palate forms from shelves of epithelia-covered mesenchyme that meet at midline and fuse. The midline epithelial seam (MES) is thought to degrade by apoptosis, epithelial-to-mesenchymal transition (EMT), or both. Failure to degrade the MES blocks fusion and