assay
≥90% (SDS-PAGE)
form
lyophilized
manufacturer/tradename
Calbiochem®
storage condition
OK to freeze
solubility
0.1 M acetic acid: soluble, water: soluble
shipped in
wet ice
storage temp.
−70°C
Quality Level
General description
Recombinant, human VEGF121 (121-amino acid splice variant of the human vascular endothelial growth factor VEGF) expressed in S. frugiperda insect cells. VEGF121 is dominantly expressed in most tumor cell lines. Forms a biological active homodimer and evidence suggests that it is also biologically active as a heterodimer with other VEGF splice variants or PIGF.
Recombinant, human VEGF121 (121-amino acid splice variant of the human vascular endothelial growth factor VEGF) expressed in S. frugiperda insect cells. VEGF121 is dominantly expressed in most tumor cell lines. Forms a biologically active homodimer and evidence suggests that it is also biologically active as a heterodimer with other VEGF splice variants or PIGF.
Biochem/physiol Actions
ED₅₀ ≥1 ng/ml as measured by the stimulation of ³H-thymidine incorporation by human umbilical vein endothelial cells and bovine aortic endothelial cells. In vivo effects using the 13-day-old chick chorioallantoic membrane (CAM) assay can be seen between 0.5-4 µg
Preparation Note
Following reconstitution, aliquot and freeze at -70°C. Stock solutions are stable for up to 3 months at -70°C.
Other Notes
Stimpfl, M., et al. 2002. Clin. Cancer Res.8, 2253.
Pufe, T., et al. 2001. Arthritis Rheum.44, 1082.
Wilting, J., et al. 1996. Dev. Biol.176, 76.
Conn, G. et al. 1990. Proc. Natl. Acad. Sci. USA87, 1323.
Pufe, T., et al. 2001. Arthritis Rheum.44, 1082.
Wilting, J., et al. 1996. Dev. Biol.176, 76.
Conn, G. et al. 1990. Proc. Natl. Acad. Sci. USA87, 1323.
Legal Information
CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
Toxicity: Standard Handling (A)
存储类别
10-13 - German Storage Class 10 to 13
Timothy R H Regnault et al.
The Journal of physiology, 550(Pt 2), 641-656 (2003-05-13)
Placental growth factor (PlGF) and vascular endothelial growth factor (VEGF) are involved in placental angiogenesis through interactions with the VEGFR-1 and VEGFR-2 receptors. The placenta of pregnancies whose outcome is fetal growth restriction (FGR) are characterized by abnormal angiogenic development
Qi Pan et al.
The Journal of biological chemistry, 282(33), 24049-24056 (2007-06-19)
Neuropilin-1 (NRP1) was first described as a receptor for the axon guidance molecule, Semaphorin3A, regulating the development of the nervous system. It was later shown that NRP1 is an isoform-specific receptor for vascular endothelial growth factor (VEGF), specifically VEGF(165). Much
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