676602
PhosphoDetect Anti-VASP (pSer²³⁹) Mouse mAb (16C2)
lyophilized, clone 16C2, Calbiochem®
别名:
Anti-Vasodilator-stimulated Phosphoprotein, Activated
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关于此项目
UNSPSC Code:
12352203
Clone:
16C2, monoclonal
Species reactivity:
mouse, human
Application:
—
Citations:
1
biological source
mouse
antibody form
purified antibody
antibody product type
primary antibodies
clone
16C2, monoclonal
form
lyophilized
contains
≤0.1% sodium azide as preservative
species reactivity
mouse, human
manufacturer/tradename
Calbiochem®
storage condition
OK to freeze
isotype
IgG1
shipped in
ambient
storage temp.
−20°C
target post-translational modification
phosphorylation (pSer239/pSer239)
Quality Level
General description
Mouse monoclonal antibody purified from serum-free cell culture supernatant by thiophilic adsorption and size exclusion chromatography. Recognizes the ~46-50 kDa VASP protein phosphorylated at Ser239.
Recognizes the ~46-50 kDa VASP protein phosphorylated at Ser239.
This PhosphoDetect Anti-VASP (pSer²³⁹) Mouse mAb (16C2) is validated for use in ELISA, Immunoblotting, ICC, IP for the detection of VASP (pSer²³⁹).
Immunogen
Human
a synthetic phosphopeptide corresponding to amino acids surrounding the Ser²³⁹ phosphorylation site of human VASP, conjugated to KLH
Application
ELISA (0.05 µg/ml)
Immunoblotting (0.1 µg/ml, chemiluminescence; 1 µg/ml, colorimetric)
Immunocytochemistry (1-10 µg/ml)
Immunoprecipitation (1-10 µg/10⁶ vanadate-treated A431 cells)
Immunoblotting (0.1 µg/ml, chemiluminescence; 1 µg/ml, colorimetric)
Immunocytochemistry (1-10 µg/ml)
Immunoprecipitation (1-10 µg/10⁶ vanadate-treated A431 cells)
Physical form
Lyophilized from 2X PBS, PEG, sucrose.
Preparation Note
Reconstitute with 1 ml of dH₂O. Following reconstitution, refrigerate (4°C) for short-term storage or aliquot, flash-freeze in liquid nitrogen, and freeze (-70°C) for long-term storage. Stock solutions are stable for up to 3 months at 4°C or for up to 1 year at -70°C. Thaw aliquots at 37°C. Avoid freeze/thaw cycles of solutions.
Analysis Note
Positive Control
Vanadate-treated A431 cells or 8-pCPT-treated platelets
Vanadate-treated A431 cells or 8-pCPT-treated platelets
Other Notes
Does not recognize the non-phosphorylated form of VASP. Suitable for monitoring the phosphorylation state of VASP Ser239 as well as PKA/PKG activity. For immunocytochemistry, SNP or 8-pCPT-cGMP treated platelets are fixed with 4% paraformaldehyde in PBS for 15 min. on ice, then permeabilized with 0.2% Triton™ X-100 detergent in PBS for 10 min at room temperature. For immunoblotting, block with 1% hemoglobin in PBS, 0.3% Triton X-100 detergent, and 0.05% Tween-20 detergent. Variables associated with assay conditions will dictate the proper working dilution.
Reinhard, M., et al. 1999. In: Guidebook to the Cytoskeletal and Motor Proteins (Eds. Kreis, T., and Vale, R.), Oxford University Press, Oxford, 168.
Schwarz, U.R., et al. 1999. Thromb. Haemost.82, 1145.
Smolenski, A., et al. 1998. J. Biol. Chem.273, 20029.
Schwarz, U.R., et al. 1999. Thromb. Haemost.82, 1145.
Smolenski, A., et al. 1998. J. Biol. Chem.273, 20029.
Legal Information
CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany
Triton is a trademark of The Dow Chemical Company or an affiliated company of Dow
Disclaimer
Toxicity: Standard Handling (A)
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存储类别
11 - Combustible Solids
wgk
WGK 1
flash_point_f
Not applicable
flash_point_c
Not applicable
Loek van Heerebeek et al.
Circulation, 126(7), 830-839 (2012-07-19)
Prominent features of myocardial remodeling in heart failure with preserved ejection fraction (HFPEF) are high cardiomyocyte resting tension (F(passive)) and cardiomyocyte hypertrophy. In experimental models, both reacted favorably to raised protein kinase G (PKG) activity. The present study assessed myocardial
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