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Merck
CN

71456

BugBuster®预混液

别名:

细胞裂解预混液

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关于此项目

NACRES:
NA.32
UNSPSC Code:
41106511

主要文件

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form

liquid

manufacturer/tradename

Novagen®

storage condition

OK to freeze

shipped in

wet ice

storage temp.

2-8°C

Quality Level

200

相关类别

Application

BugBuster®预混液的应用实例：
  • 在蛋白纯化实验中重悬和裂解细胞沉淀
  • 提取免疫印迹用蛋白
  • 作为细菌裂解缓冲液用于制备包涵体


    

Biochem/physiol Actions

BugBuster®蛋白提取试剂专门用于温和地破坏大肠杆菌细胞壁以释放可溶性蛋白。利用混合去垢剂的独特配方有效地穿破细胞壁但不会使可溶性蛋白变性。结合BugBuster试剂、rLysozyme溶液和Benzonase核酸酶，BugBuste预混液对蛋白的提取效率更高，获得的蛋白提取物下游处理更加方便。

Disclaimer

毒性：标准处理（A）

Features and Benefits

  • 为目标表达蛋白释放提供方便、快捷、高性价比的解决方案。
  • 该预混液无需稀释或进行额外的分离操作。

  • 革兰氏阳性和革兰氏阴性细菌的高效裂解
  • 最大限度回收活性可溶性蛋白
  • 能穿透细胞壁而不使可溶性蛋白变性
  • 用于制备高纯度包涵体
  • 与蛋白酶和磷酸酶抑制剂混合物兼容

General description

BugBuster®预混液可用于高效的蛋白纯化及其它相关应用。它可取代高压破菌(French press)或超声波处理等机械方法。提供两种包装规格，分别包含足以从20 g或100 g细胞糊中提取蛋白的试剂。

Legal Information

BUGBUSTER is a registered trademark of Merck KGaA, Darmstadt, Germany
Benzonase is a registered trademark of Merck KGaA, Darmstadt, Germany
NOVAGEN is a registered trademark of Merck KGaA, Darmstadt, Germany

存储类别

10 - Combustible liquids

wgk

WGK 2

flash_point_f

Not applicable

flash_point_c

Not applicable

法规信息

常规特殊物品
此项目有

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FMRP inhibits translation elongation independent of mRNA G-quadruplexes
Scarpitti MR, et al.
bioRxiv : the preprint server for biology null
Development of a synthetic biosensor for chemical exchange MRI utilizing in silico optimized peptides
Fillion AJ, et al.
Nmr in Biomedicine, 36, e5007-e5007 (2023)
Matthew Thomas Doyle et al.
Molecular cell, 81(9), 2000-2012 (2021-03-12)
The β-barrel assembly machine (BAM) integrates β-barrel proteins into the outer membrane (OM) of Gram-negative bacteria. An essential BAM subunit (BamA) catalyzes integration by promoting the formation of a hybrid-barrel intermediate state between its own β-barrel domain and that of
Daniel G Isom et al.
Proteins, 79(4), 1034-1047 (2011-03-10)
Protein thermodynamic stability is a fundamental physical characteristic that determines biological function. Furthermore, alteration of thermodynamic stability by macromolecular interactions or biochemical modifications is a powerful tool for assessing the relationship between protein structure, stability, and biological function. High-throughput approaches
Yong Y Peng et al.
Bioengineered, 5(6), 378-385 (2014-12-09)
The collagen like domain Scl2 from Streptococcus pyogenes has been proposed as a potential biomedical material. It is non-cytotoxic and non-immunogenic and can be prepared in good yield in fermentation. The Scl2 collagen domain is about a quarter of the
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