Fmoc-Gly-NovaSyn^® TGT

Pre-loaded resin for synthesis of peptide acids and protected peptide fragments containing a C-terminal glycine amino-acid residue by Fmoc SPPS.The base NovaSyn^® TG is a composite of low cross-linked polystyrene and 3000-4000 M.W. polyethylene glycol. Peptide synthesis is carried out at the ends of the PEG chains that have been functionalized with the hyper-acid labile 4-carboxytrityl alcohol linker. This use of the bulky trityl-type linker helps prevent diketopiperazine formation that can occur during piperidine treatment of Fmoc-protected dipeptidyl resins.^,,Treatment of the peptidyl resin with 20% TFE in DCM or 1% TFA in DCM cleaves the product from the resin without affecting the standard TFA-labile side-chain protecting groups. Standard TFA cleavage releases the fully deprotected peptide.


Associated Protocols and Technical Articles
Cleavage and Deprotection Protocols for Fmoc SPPS

Literature references:
^[1] K. Barlos, et al. (1989) Tetrahedron Lett., 30, 3947.
^[2] K. Barlos, et al. (1993) Ann. Chem., 215.
^[3] R. Steinauer, et al. in “Innovations & Perspectives in Solid Phase Synthesis”, R. Epton (Ed.), Mayflower Scientific Ltd., Birmingham, 1994, pp. 689.
^[4] K. Barlos & D. Gatos in “Fmoc solid phase peptide synthesis: a practical approach”, W. C. Chan & P. D.White (Eds.), Oxford University Press, Oxford, 2000, pp. 218.

Fmoc-Gly-NovaSyn^® TGT has been used to preparehydrazinopeptides as intermediates in the semi-synthesis of glycopeptides.

Color (visual): white to yellow to beige
Appearance of substance (visual): beads
Loading (Photometric determination of the Fmoc-chromophore liberated upon treatment with DBU/DMF): 0.10 - 0.30 mmol/g
Swelling Volume (in DMF): lot specific result
Identity (of the substitution): passes test
The base resin is PEG-PS-copoymer (90µm), functionalised with 4-carboxy-tritylchloride.

Replaces: 04-12-2711

NOVASYN is a registered trademark of Merck KGaA, Darmstadt, Germany

Novabiochem is a registered trademark of Merck KGaA, Darmstadt, Germany