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主要文件

NA26

Sigma-Aldrich

Anti-MSH2 (Ab-1) Mouse mAb (GB12)

liquid, clone GB12, Calbiochem®

别名:

Anti-Mismatch Repair Protein 2

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About This Item

UNSPSC代码:
12352203
NACRES:
NA.41

生物来源

mouse

质量水平

抗体形式

purified antibody

抗体产品类型

primary antibodies

克隆

GB12, monoclonal

表单

liquid

包含

≤0.1% sodium azide as preservative

种属反应性

human

请勿与下列物质发生反应

mouse

制造商/商品名称

Calbiochem®

储存条件

do not freeze

同位素/亚型

IgG1

运输

wet ice

储存温度

2-8°C

靶向翻译后修饰

unmodified

基因信息

human ... MSH2(4436)

一般描述

Purified mouse monoclonal antibody generated by immunizing BALB/c mice with the specified immunogen and fusing splenocytes with SP20 mouse myeloma cells. Recognizes the ~100 kDa MSH2 protein.
Recognizes the ~100 kDa MSH2 protein in HCT116 and SW480 cells and colon tissue.
This Anti-MSH2 (Ab-1) Mouse mAb (GB12) is validated for use in Frozen Sections, Immunoblotting, Immunoprecipitation, Paraffin Sections for the detection of MSH2 (Ab-1).

免疫原

Human
an N-terminal fragment of human MSH2

应用

Frozen Sections (5 µg/ml)

Immunoblotting (1 µg/ml)

Immunoprecipitation (1 µg/reaction)

Paraffin Sections (3 µg/ml, heat pre-treatment required)

包装

Please refer to vial label for lot-specific concentration.

警告

Toxicity: Standard Handling (A)

外形

In 50 mM sodium phosphate buffer, 0.2% gelatin, pH 7.5.

分析说明

Negative Control
LoVo cells
Positive Control
HCT116 or SW480 cells or colon tissue

其他说明

Bronner, C. E., et al. 1994. Nature368, 258.
Papadopoulos, N., et al. 1994. Science263, 1625.
Peltomäki, P. T. 1994. Annals of Medicine26, 215.
Fishel, R., et al. 1993. Cell75, 1027-1038, 1993.
Leach, F. S., et al. 1993. Cell75, 1215.
Lindbolm, A., et al. 1993. Nature Genetics5, 279.
For paraffin sections, pre-treat with heat for 20 min. Antibody should be titrated for optimal results in individual systems.

法律信息

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

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储存分类代码

10 - Combustible liquids

WGK

nwg

闪点(°F)

Not applicable

闪点(°C)

Not applicable


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