生物来源
mouse
质量水平
抗体形式
purified antibody
抗体产品类型
primary antibodies
克隆
GB12, monoclonal
表单
liquid
包含
≤0.1% sodium azide as preservative
种属反应性
human
请勿与下列物质发生反应
mouse
制造商/商品名称
Calbiochem®
储存条件
do not freeze
dilution
(Frozen Sections (5 µg/mL)
Immunoblotting (1 µg/mL)
Immunoprecipitation (1 µg/reaction)
Paraffin Sections (3 µg/mL, heat pre-treatment required))
同位素/亚型
IgG1
运输
wet ice
储存温度
2-8°C
靶向翻译后修饰
unmodified
基因信息
human ... MSH2(4436)
一般描述
Purified mouse monoclonal antibody generated by immunizing BALB/c mice with the specified immunogen and fusing splenocytes with SP20 mouse myeloma cells. Recognizes the ~100 kDa MSH2 protein.
Recognizes the ~100 kDa MSH2 protein in HCT116 and SW480 cells and colon tissue.
This Anti-MSH2 (Ab-1) Mouse mAb (GB12) is validated for use in Frozen Sections, Immunoblotting, Immunoprecipitation, Paraffin Sections for the detection of MSH2 (Ab-1).
免疫原
Human
an N-terminal fragment of human MSH2
应用
Frozen Sections (5 µg/ml)
Immunoblotting (1 µg/ml)
Immunoprecipitation (1 µg/reaction)
Paraffin Sections (3 µg/ml, heat pre-treatment required)
Immunoblotting (1 µg/ml)
Immunoprecipitation (1 µg/reaction)
Paraffin Sections (3 µg/ml, heat pre-treatment required)
包装
Please refer to vial label for lot-specific concentration.
外形
In 50 mM sodium phosphate buffer, 0.2% gelatin, pH 7.5.
分析说明
Negative Control
LoVo cells
LoVo cells
Positive Control
HCT116 or SW480 cells or colon tissue
HCT116 or SW480 cells or colon tissue
其他说明
Bronner, C. E., et al. 1994. Nature368, 258.
Papadopoulos, N., et al. 1994. Science263, 1625.
Peltomäki, P. T. 1994. Annals of Medicine26, 215.
Fishel, R., et al. 1993. Cell75, 1027-1038, 1993.
Leach, F. S., et al. 1993. Cell75, 1215.
Lindbolm, A., et al. 1993. Nature Genetics5, 279.
Papadopoulos, N., et al. 1994. Science263, 1625.
Peltomäki, P. T. 1994. Annals of Medicine26, 215.
Fishel, R., et al. 1993. Cell75, 1027-1038, 1993.
Leach, F. S., et al. 1993. Cell75, 1215.
Lindbolm, A., et al. 1993. Nature Genetics5, 279.
For paraffin sections, pre-treat with heat for 20 min. Antibody should be titrated for optimal results in individual systems.
法律信息
CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany
免责声明
Toxicity: Standard Handling (A)
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储存分类代码
10 - Combustible liquids
WGK
nwg
闪点(°F)
Not applicable
闪点(°C)
Not applicable
Leah C Young et al.
The American journal of pathology, 179(1), 411-421 (2011-06-28)
The fusion tyrosine kinase NPM-ALK is central to the pathogenesis of ALK-positive anaplastic large cell lymphoma (ALK(+)ALCL). We recently identified that MSH2, a key DNA mismatch repair (MMR) protein integral to the suppression of tumorigenesis, is an NPM-ALK-interacting protein. In
M B Weiss et al.
Cancer gene therapy, 14(1), 98-104 (2006-11-04)
The use of gene therapy to correct mutated or lost gene function for the treatment of human cancers has been an active, yet problematic area of biomedical research. Many technical difficulties, including efficient tissue-specific delivery, integration site specificity and general
Myrella Vlenterie et al.
Oncotarget, 6(33), 34680-34690 (2015-09-29)
Over 95% of all synovial sarcomas (SS) share a unique translocation, t(X;18), however, they show heterogeneous clinical behavior. We analyzed multiple SS to reveal additional genetic alterations besides the translocation. Twenty-six SS from 22 patients were sequenced for 409 cancer-related
Laure Droy-Dupré et al.
United European gastroenterology journal, 2(4), 307-314 (2014-08-02)
Villous tumours of the rectosigmoid are historically defined as broad-based lesions associated with secretory diarrhoea. This study aimed to perform a reappraisal of these tumours, on the basis of newly introduced histological, immunohistochemical and molecular parameters. For this study, 22
MutSβ abundance and Msh3 ATP hydrolysis activity are important drivers of CTG•CAG repeat expansions.
Norma Keogh et al.
Nucleic acids research, 45(17), 10068-10078 (2017-10-04)
CTG•CAG repeat expansions cause at least twelve inherited neurological diseases. Expansions require the presence, not the absence, of the mismatch repair protein MutSβ (Msh2-Msh3 heterodimer). To evaluate properties of MutSβ that drive expansions, previous studies have tested under-expression, ATPase function
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