PhosphoDetect Anti-Src Family (pTyr⁴¹⁶) Rabbit pAb

Protein A and immunoaffinity purified rabbit polyclonal antibody. Recognizes the ~60 kDa Src protein phosphorylated at Tyr⁴¹⁶.

Recognizes the ~60 kDa Src protein phosphorylated at Tyr⁴¹⁶ in COLO 201 cells and PDGF-treated NIH/3T3 cells. May also recognize other Src family kinases phosphorylated at equivalent sites.

This PhosphoDetect Anti-Src Family (pTyr⁴¹⁶) Rabbit pAb is validated for use in Immunoblotting for the detection of Src Family (pTyr⁴¹⁶).

Human

a synthetic phosphopeptide corresponding to amino acids surrounding the Tyr⁴¹⁶ phosphorylation site of human Src

Immunoblotting (1:1000)

Toxicity: Standard Handling (A)

In 150 mM NaCl, 10 mM sodium HEPES, 100 µg/ml BSA, 50% glycerol, pH 7.5.

Do not aliquot.

Positive Control
COLO 201 cells, PDGF-treated NIH/3T3 cells, and breast carcinoma

May also recognize other Src family kinases phosphorylated at equivalent sites. The immunogen sequence is 100% conserved in chicken, rat, and Xenopus, but cross-reactivity has not been tested. For immunoblotting the membrane should be incubated with antibody diluted in 5% BSA, 1X TBS, 0.1% Tween^®-20 detergent at 4°C overnight with gentle shaking. Antibody should be titrated for optimal results in individual systems.

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