生物来源
rabbit
质量水平
偶联物
unconjugated
抗体形式
serum
抗体产品类型
primary antibodies
克隆
polyclonal
种属反应性
rat, mouse
制造商/商品名称
Chemicon®
技术
ELISA: suitable
western blot: suitable
NCBI登记号
UniProt登记号
运输
wet ice
靶向翻译后修饰
unmodified
基因信息
mouse ... Slc2A3(20527)
rat ... Slc2A3(25551)
免疫原
Synthetic peptide corresponding to the C-terminus of mouse Glut-3 coupled to KLH. Product sold sold as AG617
应用
Anti-Glucose Transporter GLUT-3 Antibody, C-terminus is an antibody against Glucose Transporter GLUT-3 for use in ELISA & WB.
Western blotting: 1:1,000-1:5,000, using chemiluminescent detection. Detects a 45-48kDa band in membrane preps from rat brain. Slight differences in band sizes in different tissues; reported range is 45-55kDa.
Other detection methods may require a higher concentration of antibody.
ELISA: 1:10,000 - 1:50,000, using 50-100 ng of antigen/well.
Optimal working dilutions must be determined by end user.
Other detection methods may require a higher concentration of antibody.
ELISA: 1:10,000 - 1:50,000, using 50-100 ng of antigen/well.
Optimal working dilutions must be determined by end user.
生化/生理作用
Recognizes Glut-3 in mouse and rat tissues. C terminal peptide used shows no homology to the human sequence.
外形
Liquid rabbit antiserum containing 0.05% sodium azide.
制备说明
Store at -20°C for up to 12 months after date of receipt in undiluted aliquots. Avoid repeated freeze-thaw cycles.
分析说明
Control
The control peptide is available for absorbtion studies. Please see catalog number AG617.
The control peptide is available for absorbtion studies. Please see catalog number AG617.
法律信息
CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany
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储存分类代码
10 - Combustible liquids
WGK
WGK 1
Sze Ting Cecilia Kwan et al.
The Journal of nutrition, 147(11), 2083-2092 (2017-09-22)
Background: Fetal growth is dependent on placental nutrient supply, which is influenced by placental perfusion and transporter abundance. Previous research indicates that adequate choline nutrition during pregnancy improves placental vascular development, supporting the hypothesis that choline may affect placental nutrient
C M Cheng et al.
Proceedings of the National Academy of Sciences of the United States of America, 97(18), 10236-10241 (2000-08-24)
The brain has enormous anabolic needs during early postnatal development. This study presents multiple lines of evidence showing that endogenous brain insulin-like growth factor 1 (Igf1) serves an essential, insulin-like role in promoting neuronal glucose utilization and growth during this
Francesca Gaccioli et al.
Biology of reproduction, 89(4), 96-96 (2013-09-06)
The mammalian target of rapamycin (mTOR) and the eukaryotic initiation factor 2 (eIF2) signaling pathways control protein synthesis in response to nutrient availability. Moreover, mTOR is a positive regulator of placental nutrient transport and is involved in the regulation of
Raimund I Herzog et al.
The Journal of clinical investigation, 123(5), 1988-1998 (2013-04-02)
Hypoglycemia occurs frequently during intensive insulin therapy in patients with both type 1 and type 2 diabetes and remains the single most important obstacle in achieving tight glycemic control. Using a rodent model of hypoglycemia, we demonstrated that exposure to
Cinzia Dello Russo et al.
The Journal of biological chemistry, 278(8), 5828-5836 (2002-12-18)
Activation of peroxisome proliferator-activated receptors (PPARs) can regulate brain physiology and provide protection in models of neurological disease; however, neither their exact targets nor mechanisms of action in brain are known. In many cells, PPAR gamma agonists increase glucose uptake
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