biological source
rabbit
Quality Segment
conjugate
unconjugated
antibody form
serum
antibody product type
primary antibodies
clone
polyclonal
species reactivity
rat, mouse
manufacturer/tradename
Chemicon®
technique(s)
ELISA: suitable, western blot: suitable
NCBI accession no.
UniProt accession no.
shipped in
wet ice
target post-translational modification
unmodified
Gene Information
mouse ... Slc2A3(20527)
rat ... Slc2A3(25551)
Immunogen
Synthetic peptide corresponding to the C-terminus of mouse Glut-3 coupled to KLH. Product sold sold as AG617
Application
Anti-Glucose Transporter GLUT-3 Antibody, C-terminus is an antibody against Glucose Transporter GLUT-3 for use in ELISA & WB.
Western blotting: 1:1,000-1:5,000, using chemiluminescent detection. Detects a 45-48kDa band in membrane preps from rat brain. Slight differences in band sizes in different tissues; reported range is 45-55kDa.
Other detection methods may require a higher concentration of antibody.
ELISA: 1:10,000 - 1:50,000, using 50-100 ng of antigen/well.
Optimal working dilutions must be determined by end user.
Other detection methods may require a higher concentration of antibody.
ELISA: 1:10,000 - 1:50,000, using 50-100 ng of antigen/well.
Optimal working dilutions must be determined by end user.
Biochem/physiol Actions
Recognizes Glut-3 in mouse and rat tissues. C terminal peptide used shows no homology to the human sequence.
Physical form
Liquid rabbit antiserum containing 0.05% sodium azide.
Preparation Note
Store at -20°C for up to 12 months after date of receipt in undiluted aliquots. Avoid repeated freeze-thaw cycles.
Analysis Note
Control
The control peptide is available for absorbtion studies. Please see catalog number AG617.
The control peptide is available for absorbtion studies. Please see catalog number AG617.
Legal Information
CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany
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存储类别
10 - Combustible liquids
wgk
WGK 1