technique(s)
immunocytochemistry: suitable, immunohistochemistry: suitable, western blot: suitable
biological source
rabbit
antibody form
serum
antibody product type
primary antibodies
clone
polyclonal
species reactivity
human
manufacturer/tradename
Chemicon®
NCBI accession no.
UniProt accession no.
shipped in
wet ice
target post-translational modification
unmodified
Gene Information
human ... ATG10(83734)
General description
Autophagy is the process by which cells recycle cytoplasm and dispose of excess or defective organelles. This process is suggested to be involved development, differentiation, growth regulation and tissue remodeling in multicellular organisms. The APG10 protein plays a role in the conjugation of APG5 to APG12 and this conjugate is required for elongation of the isolation membrane to form a complete spherical autophagosome. The APG10 is able to interact with MAP1LC3A and with APG5 and APG7.
Immunogen
Synthetic peptide corresponding to a region within the C-terminus of human APG10 protein.
Application
Research Category
Neuroscience
Neuroscience
Research Sub Category
Neurodegenerative Diseases
Neurodegenerative Diseases
Anti-APG10 Antibody detects level of APG10 & has been published & validated for use in WB, IC, IH.
Western blot: 1:200-1:1,000
Immunocytochemistry: 1:200-1:1,000
Immunohistochemistry: 1:200-1:1,000
Optimal working dilutions must be determined by end user.
Immunocytochemistry: 1:200-1:1,000
Immunohistochemistry: 1:200-1:1,000
Optimal working dilutions must be determined by end user.
Biochem/physiol Actions
APG10 [Autophagy-related protein 10, APG10-like, ATG10].
Physical form
Lyophilized. Reconstitute with 50 μL of sterile distilled water. Centrifuge to remove any insoluble material. Contains no preservative.
Serum
Preparation Note
Maintain lyophilized material frozen at -20°C to -70°C for up to 12 months after date of receipt. After reconstitution maintain at -20°C in undiluted aliquots for up to 6 months. Avoid repeated freeze/thaw cycles. Glycerol (1:1, ACS or better grade) can be added for additional stability.
Legal Information
CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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存储类别
10 - Combustible liquids
相关内容
Research Focus: Autophagy
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