Anti-Vesicular Acetylcholine Transporter Antibody

Synthetic peptide corresponding to the C-terminus of the predicted rat VAChT protein.

Immunohistochemistry: 1:500-1:1,500 on 4% paraformaldehyde fixed tissue. Sections were cut at 15 μm with a cryostat or 30 μm with a sliding microtome.

Immunoblotting

Blocking peptide available (Catalog Number AG223).

Optimal working dilutions must be determined by the end user.

Research Category
Neuroscience

Research Sub Category
Ion Channels & Transporters

Neuronal & Glial Markers

This Anti-Vesicular Acetylcholine Transporter Antibody is validated for use in IH, WB for the detection of Vesicular Acetylcholine Transporter.

Vesicular Acetylcholine Transporter (VAChT). By Western blot the antibody reacted with a single band in rat brain homogenates. This band corresponded to the size of the predicted rVAChT. There was no staining of blots incubated with the antibody that had been preabsorbed with the peptide used for antibody production. By immunohistochemistry the staining was strong in the inner retina where cholinergic processes are located and weak in cholinergic cell bodies. There was no background staining noted. In the CNS staining was strong in fibers and stains cholinergic cell bodies well.

Affinity purified immunoglobulin. Lyophilized from 0.1 M PBS with 1.0% BSA and 0.1% sodium azide. Reconstitute with 100 μL of sterile distilled water.

Maintain lyophilized material at 2-8°C for up to 12 months. Maintain reconstituted material at -20°C in undiluted aliquots for up to 6 months. Avoid repeated freeze/thaw cycles.

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.