Anti-Integrin α4 Antibody, PhosphoSerine 988

Integrin alpha4 has been implicated in the pathogenesis of diseases such as asthma, arthritis, and multiple sclerosis. Phosphorylation of the cytosolic tail of integrin alpha4 at position 988 is required for optimal alpha4beta1 integrin-mediated directed cell migration. In the absence of phosphorylation at Ser988, the cytosolic tail of integrin alpha4 binds tightly to the signaling adaptor protein, paxillin, inhibiting cell migration. Phosphorylation of alpha4 at Ser988 promotes dissociation from paxillin thereby enhancing cell migration (Han, 2003). MW is approximately 150 kDa.

Epitope: PhosphoSerine 988

Synthetic peptide corresponding to the cytoplasmic tail of human integrin alpha4 (a.a. 986-995) containing a phosphorylated serine at position 988. The phosphopeptide was conjugated to KLH using gluteraldehyde.

ELISA: 1:10,000 (direct)

Western blot: 1:1,000 to 1:5,000. On western blots of Jurkat cell extracts the antibody recognizes a major band at 140 kDa corresponding to Integrin alpha4 as well as 3 weak bands at approximately 60 kDa that are believed to be non-specific.

Immunoprecipitation: 5 μL per 500 μg whole Jurkat cell lysate.

NOTE: the use of specific inhibitors of phosphoserine phosphatse activity such as okadaic acid or NaF is strongly recommended during the preparation of protein extracts for western blot and immunoprecipitation.

Optimal working dilutions must be determined by end user.

Research Category
Cell Structure

Research Sub Category
Integrins

This Anti-Integrin α4 Antibody, PhosphoSerine 988 is validated for use in ELISA, IP, WB for the detection of Integrin α4.

Integrin alpha4 phosphorylated on serine 988. Does not react with integrin alpha4 that is not phosphorylated at serine 988 (Han, 2001).

Liquid rabbit serum containing no preservative.

Maintain at -20°C in undiluted aliquots for up to 12 months from date of receipt. Avoid repeated freeze/thaw cycles.

Control
POSITIVE CONTROL: Jurkat cells

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