Anti-N-WASP Antibody

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Anti-N-WASP Antibody is an antibody against N-WASP for use in ELISA & WB.

Research Category
Signaling

Research Sub Category
Cytoskeletal Signaling

Western blot: 1:1000 ELISA: 1:2000

This antibody detects a 65 kDa protein corresponding to the molecular weight of N-WASP on SDS-PAGE immunoblots of neonatal rat brain lysate. It is also detects 65 kDa proteins in A431, human endothelial, and SKN-SH cells. It does not recognize the 63 kDa WASP protein in Jurkat cell lysate.

Members of the Wiskott-Aldrich sydrome protein (WASP) family regulate the formation of actin-based cell structures in many cell types. These proteins contain C-terminal actin-binding domains that can stimulate actin polymerization. In addition, these proteins bind the ARP2/3 complex, which can nucleate actin polymerization at sites that lead to branched actin structures. WASP is expressed primarily in hematopoietic cells, while its homolog N-WASP is widely expressed. These proteins have 48% identity in human with the highest homology in the functional regions of these proteins. Serine and tyrosine phosphorylation regulates the activity of both proteins. WASP is observed as a 63 kDa protein in hematopoietic cells, while N-WASP is observed as a 65 kDa in many tissues, especially brain.

Synthetic peptide (coupled to KLH) corresponding to amino acid residues in the N-terminus of human N-WASP. This N-WASP peptide sequence is 100% homologous to rat and mouse N-WASP, and has low homology to the corresponding region in the human WASP sequence.

Affinity-purified antibody is supplied in 100 μl phosphate-buffered saline, 50% glycerol, 1 mg/ml BSA, and 0.05% sodium azide.

Maintain at -20°C for up to one year from date of receipt. Do not aliquot.

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany