Collagen Type II extracted and purified from mouse fetal cartilage.

Anti-Collagen Type II Antibody is an antibody against Collagen Type II for use in ELISA, IF, RIA, IH.

Immunohistochemistry: 1:40-1:80 dilution for immunofluorescent staining of fresh frozen mouse articular tissue. Acetone or methyl-carnoy fixation is also reactive.

Radioimmunoassay

ELISA

Optimal working dilutions must be determined by end user.

Research Category
Cell Structure

Research Sub Category
ECM Proteins

Antibody shows by RIA cross reactivity with mouse collagen types I, III, IV of less than 0.1% at a dilution of 1:100. The antibody also demonstrates a reactivity with mouse fibronectin of less than 0.1% by RIA at dilution of 1:100. There is also cross reactivity with bovine collagen type II by ELISA

Format: Purified

Protein G affinity purified IgG fraction at 1.0 mg/mL in 0.01M phosphate, 0.09M NaCl, pH 7.2 with no preservatives.

Maintain frozen at -20°C for up to 12 months from date of receipt. Avoid repeated freeze/thaw cycles.

Important Note: During shipment, small volumes of product will occasionally become entrapped in the seal of the product vial. For products with volumes of 200 μL or less, we recommend gently tapping the vial on a hard surface or briefly centrifuging the vial in a tabletop centrifuge to dislodge any liquid in the container′s cap.

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.