产品名称
Anti-Collagen Type II Antibody, Chemicon®, from rabbit
biological source
rabbit
antibody form
affinity purified immunoglobulin
antibody product type
primary antibodies
clone
polyclonal
species reactivity
bovine, mouse
manufacturer/tradename
Chemicon®
technique(s)
ELISA: suitable
immunofluorescence: suitable
immunohistochemistry: suitable
radioimmunoassay: suitable
UniProt accession no.
shipped in
dry ice
target post-translational modification
unmodified
Gene Information
mouse ... Col2A1(12824)
Application
Anti-Collagen Type II Antibody is an antibody against Collagen Type II for use in ELISA, IF, RIA, IH.
Immunohistochemistry: 1:40-1:80 dilution for immunofluorescent staining of fresh frozen mouse articular tissue. Acetone or methyl-carnoy fixation is also reactive.
Radioimmunoassay
ELISA
Optimal working dilutions must be determined by end user.
Radioimmunoassay
ELISA
Optimal working dilutions must be determined by end user.
Research Category
Cell Structure
Cell Structure
Research Sub Category
ECM Proteins
ECM Proteins
Biochem/physiol Actions
Antibody shows by RIA cross reactivity with mouse collagen types I, III, IV of less than 0.1% at a dilution of 1:100. The antibody also demonstrates a reactivity with mouse fibronectin of less than 0.1% by RIA at dilution of 1:100. There is also cross reactivity with bovine collagen type II by ELISA
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Immunogen
Collagen Type II extracted and purified from mouse fetal cartilage.
Other Notes
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
Physical form
Format: Purified
Protein G affinity purified IgG fraction at 1.0 mg/mL in 0.01M phosphate, 0.09M NaCl, pH 7.2 with no preservatives.
Preparation Note
Maintain frozen at -20°C for up to 12 months from date of receipt. Avoid repeated freeze/thaw cycles.
Important Note: During shipment, small volumes of product will occasionally become entrapped in the seal of the product vial. For products with volumes of 200 μL or less, we recommend gently tapping the vial on a hard surface or briefly centrifuging the vial in a tabletop centrifuge to dislodge any liquid in the container′s cap.
Important Note: During shipment, small volumes of product will occasionally become entrapped in the seal of the product vial. For products with volumes of 200 μL or less, we recommend gently tapping the vial on a hard surface or briefly centrifuging the vial in a tabletop centrifuge to dislodge any liquid in the container′s cap.
Legal Information
CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany
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存储类别
12 - Non Combustible Liquids
wgk
WGK 2
flash_point_f
Not applicable
flash_point_c
Not applicable
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