biological source
rabbit
Quality Level
conjugate
unconjugated
antibody form
affinity purified immunoglobulin
antibody product type
primary antibodies
clone
polyclonal
purified by
affinity chromatography
species reactivity
Xenopus, canine, rabbit, sheep, monkey, human, mouse, rat
manufacturer/tradename
Chemicon®
technique(s)
ELISA: suitable, immunohistochemistry: suitable, immunoprecipitation (IP): suitable, western blot: suitable
NCBI accession no.
UniProt accession no.
shipped in
wet ice
target post-translational modification
unmodified
Gene Information
human ... MAP2K5(5607)
mouse ... Map2K5(23938)
Immunogen
Human MEK 5 synthetic peptide (aa. 59-74; Genbank Accession # U71088), conjugated to KLH; identical to rat MEK5 sequence over these residues.
Application
Anti-MEK5 Antibody is an antibody against MEK5 for use in ELISA, IH, IP & WB.
Research Category
Signaling
Signaling
Research Sub Category
MAP Kinases
MAP Kinases
Western Blot: 1 μg/mL
Immunohistochemistry: 1:1,000
Immunoprecipitation
EIA
Optimal working dilutions must be determined by end user.
Immunohistochemistry: 1:1,000
Immunoprecipitation
EIA
Optimal working dilutions must be determined by end user.
Biochem/physiol Actions
MEK 5 is also known as MAP Kinase Kinase 5, or ERK Kinase 5. AB3184 detects a 49 kDa protein, corresponding to the expeted molecular mass of Mek5, in rat brain, rat liver, rat kidney, rat heart, mouse brain, and mouse testis tissue extracts as well as in cell lysates prepared from the rat pheochromacytoma (PC12) and the human HeLa cell lines. No cross-reactivity with MEK 1, 2, 3, 4 or 6 has been observed with this antibody.
Physical form
Affinity purified immunoglobulin. Affinity purified immunoglobulin
Liquid containing PBS, pH 7.2. No preservatives.
Liquid containing PBS, pH 7.2. No preservatives.
Preparation Note
Maintain frozen at -20°C in undiluted aliquots for up to 12 months from date of receipt. Thawed product may be stored at 2-8°C for up to 4 weeks. Avoid repeated freeze/thaw cycles.
Other Notes
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
Legal Information
CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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wgk
WGK 2
flash_point_f
Not applicable
flash_point_c
Not applicable
存储类别
12 - Non Combustible Liquids
Fahima Munmun et al.
Journal of pineal research, 73(2), e12814-e12814 (2022-06-09)
Melatonin, the primary hormone involved in circadian entrainment, plays a significant role in bone physiology. This study aimed to assess the role of MEK1/2 and MEK5 in melatonin-mediated actions in mouse and human mesenchymal stem cells (MSCs) and on bone
Pamela A Lochhead et al.
Cell cycle (Georgetown, Tex.), 15(4), 506-518 (2016-03-10)
ERK5, encoded by MAPK7, has been proposed to play a role in cell proliferation, thus attracting interest as a cancer therapeutic target. While oncogenic RAS or BRAF cause sustained activation of the MEK1/2-ERK1/2 pathway, ERK5 is directly activated by MEK5.
Pamela A Lochhead et al.
Nature communications, 11(1), 1383-1383 (2020-03-15)
The dual protein kinase-transcription factor, ERK5, is an emerging drug target in cancer and inflammation, and small-molecule ERK5 kinase inhibitors have been developed. However, selective ERK5 kinase inhibitors fail to recapitulate ERK5 genetic ablation phenotypes, suggesting kinase-independent functions for ERK5.
全球贸易项目编号
| 货号 | GTIN |
|---|---|
| AB3184 | 04053252508646 |