产品名称
Anti-T7 Epitope Tag Antibody, T7 (MASMTGGQQMG), Chemicon®, from rabbit
biological source
rabbit
antibody form
affinity purified immunoglobulin
clone
polyclonal
purified by
affinity chromatography
species reactivity (predicted by homology)
all
manufacturer/tradename
Chemicon®
technique(s)
immunocytochemistry: suitable
immunofluorescence: suitable
immunoprecipitation (IP): suitable
western blot: suitable
shipped in
wet ice
target post-translational modification
unmodified
Application
Anti-T7 Epitope Tag Antibody, T7 (MASMTGGQQMG) detects level of T7 Epitope Tag & has been published & validated for use in IP, WB & IC.
Research Category
Epitope Tags & General Use
Epitope Tags & General Use
Research Sub Category
Epitope Tags
Epitope Tags
Western blot: 1:1,000-1:10,000 colorimetric; 1:1,000-1:30,000 ECL.
Immunocytochemistry: 1:100-1:400
ELISA: 1:100-1:500 for coating; 1:1,000-1:30,000 for primary.Immunofluorescence-suitable
Immunoprecipitation: 2-5μg/mL in 300-500μL of cell lysate (150-300μg/mL total protein). Lysis buffer 0.5% triton X-100 with proteinase inhibitors. Use high quality protein A beads or anti-rabbit conjugated beads for best results. Run bead-only (no T7 antibody) controls to mark bands related to protein A breakdown products.
Optimal working dilutions must be determined by the end user.
Immunocytochemistry: 1:100-1:400
ELISA: 1:100-1:500 for coating; 1:1,000-1:30,000 for primary.Immunofluorescence-suitable
Immunoprecipitation: 2-5μg/mL in 300-500μL of cell lysate (150-300μg/mL total protein). Lysis buffer 0.5% triton X-100 with proteinase inhibitors. Use high quality protein A beads or anti-rabbit conjugated beads for best results. Run bead-only (no T7 antibody) controls to mark bands related to protein A breakdown products.
Optimal working dilutions must be determined by the end user.
Biochem/physiol Actions
T7 (MASMTGGQQMG). Rabbits were immunized with T7 (MASMTGGQQMG) conjugated to KLH. Antibody was isolated by affinity chromatography using the peptide immobilized on solid support.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Immunogen
Epitope: T7 (MASMTGGQQMG)
MASMTGGQQMG (T7) conjugated to KLH
Other Notes
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
Physical form
Affinity purified immunoglobulin. Liquid in PBS, pH 7.2 with 0.1% sodium azide.
Preparation Note
Maintain at 2-8°C in undiluted for up to 6 months from date of receipt.
Legal Information
CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany
存储类别
12 - Non Combustible Liquids
wgk
WGK 2
flash_point_f
Not applicable
flash_point_c
Not applicable
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