Anti-Smad2 Antibody, phospho-specific (Ser465/467)

SMAD2 or Mothers against decapentaplegic homolog 2 is a polypeptide that, as its name describes, is a homolog of the Drosophila gene: "Mothers against decepentaplegic". It belongs to the SMAD family of proteins, which belong to the TGF-β superfamily of modulators. Like many other TGF-β family members SMAD2 is involved in cell signaling. SMAD2 modulates signals of activin and TGF-β′s. It interacts with SMAD anchor for receptor activation (SARA). The binding of ligands causes the phosphorylation of the SMAD2 protein and the dissociation from SARA and the association with SMAD4. It is subsequently transferred to the nucleus where it forms complexes with other proteins and acts as a transcription factor. SMAD2 is a receptor regulated SMAD (R-SMAD) and is activated by bone morphogenetic protein type 1 receptor kinase.

~58 kDa

Epitope: Phospho-Ser465/467

KLH-conjugated, synthetic phospho-peptide corresponding to residues surrounding Ser465/467 of human Smad2.

Research Category
Epigenetics & Nuclear Function

Research Sub Category
Transcription Factors

Use Anti-Smad2 Antibody, phospho-specific (Ser465/467) (Rabbit Polyclonal Antibody) validated in ICC, IF, IHC(P), WB to detect Smad2 also known as MAD-mothers against decapentaplegic-homolog 2.

Western Blotting:
1:1,000 dilution of a previous lot was used. For best results, incubate membrane with diluted antibody in 5% w/v nonfat dry milk, 1X TBS, 0.1% Tween-20 at 4°C overnight with gentle agitation.

Immunohistochemistry (Paraffin):
1:2000 dilution of a previous lot was used.

Immunocytochemistry (IF):
1:500 dilution of a previous lot was used.

Optimal working dilutions must be determined by the end user.

By western blot, the antibody recognizes a band migrating at approximately 58 kDa corresponding to Smad2 only when dually phosphorylated at Ser465 and Ser467. The antibody may also detect phosphorylated Smad3 (Ser423 and Ser425). This antibody does not c

Purified rabbit polyclonal in buffer containing 10 mM sodium HEPES, pH 7.5, 150 mM NaCl, 100 μg/mL BSA, with 50% glycerol.

Stable for 1 year at -20ºC from date of receipt.

Control
POSITIVE CONTROL: TGF-β treated HepG2 cells.

Concentration:Please refer to the Certificate of Analysis for the lot-specific concentration.

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