Anti-CHAPSYN-110 Antibody

GST fusion protein (Smith & Johnson 1988) and of rat chapsyn-110 (amino acids 336-379) (Brenman et al. 1996; Kim et al. 1996).

This Anti-CHAPSYN-110 Antibody is validated for use in WB, IH for the detection of CHAPSYN-110.

Western blot: 1 to 3 μg/mL (1:300-1:1000) using ECL

Dilutions should be made using a carrier protein such as BSA (1-3%)

Optimal working dilutions must be determined by the end user.

Recognizes a full length chapsyn-110 protein. It has exhibited no cross reactivity with other known related proteins tested so far.

The immunogen sequence is 42 of 44 amino acids identical in human.

Format: Purified

Purified immunoglobulin by Protein A. Lyophilized from phosphate buffered saline, pH 7.4, containing 1% BSA, 5% sucrose as a stabilizer and 0.025% sodium azide as a preservative. Reconstitute with 200 μL of sterile deionized water. Centrifuge antibody preparation before use (10,000 xg for 5 min).

Control
CONTROL ANTIGEN: Included free of charge with the antibody is 50 μg of control antigen (lyophilized powder in phosphate buffered saline, pH 7.4, containing 5% sucrose and 0.025% sodium azide). The stock solution of the antigen can be made up using 200 μL of sterile deionized water. For positive control, in Western blot using 10 ng of protein per lane. For negative control, preincubate 1 μg of fusion protein with 1 μg of antibody for one hour at room temperature. Optimal concentrations must be determined by the end user.

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

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