Anti-Homocysteine Antibody

Homocysteine a sulphur containing amino acid, is formed by the transmethylation of methionine, an essential amino acid. Homocysteine may be irreversibly metabolised to cystathionine by cystathionine synthetase. This reaction uses pyridoxal phosphate (Vitamin B6) as a co-enzyme (see figure). The enzyme cystathionine synthetase is deficient in homocysteinuria, a rare genetic disease associated with high blood levels of homocysteine and the development of premature atherosclerosis. This and the association of other rare enzyme deficiencies with homocysteinemia and premature atherosclerosis led McCully to formulate the homocysteine hypothesis of atherosclerosis (McCully KS, The homocysteine theory of atherosclerosis. Atherosclerosis. 1976; 20: 23-27)

Homocysteine-Glutaraldehyde-BSA

Research Category
Neuroscience

Research Sub Category
Neurofilament & Neuron Metabolism

Neurodegenerative Diseases

Suggested working dilution: 1:1,000-1:5,000

Optimal working dilutions must be determined by the end user.

This Anti-Homocysteine Antibody is validated for use in ELISA for the detection of Homocysteine.

Homocysteine



The cross-reactivities were determined using an ELISA test by competition experiments with the following compounds:



Compound Cross-reactivity ratio

Homocysteine-G-BSA 1

Cysteine-G-BSA 1/450

Homocysteic acid-G-BSA 1/>100,000



Abbreviations:



(G) Glutaraldehyde

(BSA) Bovine Serum Albumin

Rabbit antiserum. Liquid with 0.05% sodium azide.

Maintain at -20°C in undiluted aliquots for up to 6 months after date of receipt. Avoid repeated freeze/thaw cycles.

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.