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Merck
CN

AB5609

Sigma-Aldrich

Anti-GABA A Receptor α1 Antibody, cytoplasmic loop

Chemicon®, from rabbit

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关于此项目

UNSPSC代码:
12352203
eCl@ss:
32160702
NACRES:
NA.41
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生物来源

rabbit

偶联物

unconjugated

抗体形式

affinity purified immunoglobulin

抗体产品类型

primary antibodies

克隆

polyclonal

纯化方式

affinity chromatography

种属反应性

rat

制造商/商品名称

Chemicon®

技术

immunoprecipitation (IP): suitable
western blot: suitable

NCBI登记号

UniProt登记号

运输

dry ice

靶向翻译后修饰

unmodified

基因信息

human ... GABRA1(2554)

免疫原

Epitope: cytoplasmic loop
Fusion protein from the cytoplasmic loop of rat GABAA receptor alpha1 subunit.

应用

Anti-GABA A Receptor α1 Antibody, cytoplasmic loop is an antibody against GABA A Receptor α1 for use in IP & WB.
Research Category
Neuroscience
Research Sub Category
Neurotransmitters & Receptors
Western blot: 1:1,000

Immunopreciptation: The antibody will immunoprecipitate 59% of all GABA A receptors in dodecysulfate extracts of rat forebrains (150 μL) using 15 μg of antibody.

Optimal working dilutions must be determined by the end user.

生化/生理作用

GABAA receptor alpha1 subunit, cytoplasmic loop. The apparent molecular mass of 51 kDa in rat brain membrane fractions. The antibody shows no reactivity in the forebrain of alpha1 knockout mice by Western blot.

外形

Affinity purified immunoglobulin. Liquid in 10 mM Hepes, pH 7.5, with 150 mM NaCl, 100 μg/mL BSA and 50% glycerol. Approximate concentration is 0.15 mg/mL.

制备说明

Maintain at -20°C in undiluted for up to 6 months after date of receipt. Avoid repeated freeze/thaw cycles. Do not store in a self-defrosting freezer.

法律信息

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

免责声明

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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储存分类代码

10 - Combustible liquids

WGK

WGK 2


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Stephanie M Miller et al.
Developmental neuroscience, 39(5), 375-385 (2017-05-05)
GABA is a major neurotransmitter in the mammalian brain. In the mature brain GABA exerts inhibitory actions via the GABAA receptor (GABAAR); however, in the immature brain GABA provides much of the excitatory drive. We examined the expression of 3
Stephanie M Miller et al.
Journal of neurochemistry, 139(3), 471-484 (2016-10-25)
Seizures are a common manifestation of hypoxic-ischaemic brain injury in the neonate. In status epilepticus models alterations to GABAA R subunit expression have been suggested to contribute to (i) abnormal development of the GABAergic system, (ii) why seizures become self-sustaining
Stephanie M Miller et al.
Brain structure & function, 223(2), 1025-1033 (2017-12-29)
The GABAA receptor provides the majority of inhibitory neurotransmission in the adult central nervous system but in immature brain is responsible for much of the excitatory drive, a requirement for normal brain development. It is well established that GABAA receptor

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