Anti-Dab1 Antibody

80 kDa

Dab1 is a protein arising from the mouse gene dab, that is related to the Drosophila gene ′disabled′. Mutations in the mouse dab gene may result in the ′scrambler′ and ′yotari′ phenotypes. Dab encodes a phosphoprotein that binds non-receptor tyrosine kinases and has been implicated in neuron development. Dab1 is expressed in neuronal populations exposed to reelin, as determined by in situ hybridization to embryonic mouse brain tissue. Dab1 may function as a signaling molecule that regulates positioning in the developing brain. Cloning of human DAB1 and sequence determinations suggests a 96% homology to the mouse sequence. Very low density lipoprotein receptors (VLDLR) and apolipoprotein E receptor-2 (APOER2) both bind Dab1 on their cytoplasmic tails. Dab1 expression is upregulated in knockout mice lacking both Vldlr and Apoer2 genes.

Highly purified GST-fusion protein corresponding to residues 400-555 of mouse Dab1.

Research Category
Neuroscience

Research Sub Category
Developmental Neuroscience

Signaling Neuroscience

This Anti-Dab1 Antibody is validated for use in IC, IH, IP, WB for the detection of Dab1.

Western blotting: 1:1,000-1:5,000

Immunocytochemistry: 1:500-1:1,000

Immunohistochemistry: 1:5,000-1:20,000 on cryofixed tissues

Immunoprecipitation

Optimal working dilutions must be determined by the end user.

On western blots of mouse brain extracts, the antibody recognizes a band migrating at approximately 80 kDa corresponding to Dab1. No cross reactivity to human or mouse Dab2.

Delipidated and defibrinated Serum in 0.02M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2 with 0.01% sodium azide.

Serum, delipidated and defibrinated

Maintain for 1 year at -20°C from date of shipment. Aliquot to avoid repeated freezing and thawing. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Control
Brain tissue

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Replaces: MABS167

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.