生物来源
rabbit
抗体形式
purified immunoglobulin
抗体产品类型
primary antibodies
克隆
polyclonal
种属反应性
bovine
制造商/商品名称
Chemicon®
技术
ELISA: suitable
immunohistochemistry (formalin-fixed, paraffin-embedded sections): suitable
radioimmunoassay: suitable
适用性
not suitable for Western blot
NCBI登记号
UniProt登记号
运输
dry ice
基因信息
human ... COL1A1(1277)
免疫原
从纯化的牛皮肤中提取的纯化的牛I型胶原。
应用
免疫组化: 1:40稀释用于冷冻牛肝和皮肤组织的免疫荧光染色。
在2%福尔马林或PFA固定,石蜡包埋的组织上进行免疫组化需要HEIR抗原修复和增强的酶法检测;甲基卡诺、丙酮或酸醛固定可增强石蜡切片反应性。
放射免疫测定
ELISA:1:200定向
不推荐用于蛋白质印迹。
在2%福尔马林或PFA固定,石蜡包埋的组织上进行免疫组化需要HEIR抗原修复和增强的酶法检测;甲基卡诺、丙酮或酸醛固定可增强石蜡切片反应性。
放射免疫测定
ELISA:1:200定向
不推荐用于蛋白质印迹。
抗I型胶原抗体可检测I型胶原水平&已发表&经验证可用于ELISA、RIA、IH、IH(P)。
研究子类别
ECM 蛋白
ECM 蛋白
研究类别
细胞结构
细胞结构
生化/生理作用
与牛I型胶原反应。
在1:500稀释的RIA中,抗体与II型,IX &XI型牛胶原或纤连蛋白的交叉反应性低于0.1%;其他胶原蛋白和ECM蛋白未经测试。
与人I型胶原的反应性<2.0%,RIA,1:500;通过RIA与I型鸡胶原的反应性在1:500稀释度下小于0.1%。
在1:500稀释的RIA中,抗体与II型,IX &XI型牛胶原或纤连蛋白的交叉反应性低于0.1%;其他胶原蛋白和ECM蛋白未经测试。
与人I型胶原的反应性<2.0%,RIA,1:500;通过RIA与I型鸡胶原的反应性在1:500稀释度下小于0.1%。
外形
形式:纯化
蛋白G纯化的IgG部分,溶于0.01 M磷酸盐,0.09 M NaCl,pH值7.2中1 mg/mL的液体。无防腐剂。
制备说明
在-20°C下可冷冻保存长达12个月。 应避免反复冻/融循环
其他说明
替代品:AB749
浓度:请参考批次特异性浓缩物的分析证书。
法律信息
CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany
免责声明
除非我们的产品目录或产品附带的其他公司文档另有说明,否则我们的产品仅供研究使用,不得用于任何其他目的,包括但不限于未经授权的商业用途、体外诊断用途、离体或体内治疗用途或任何类型的消费或应用于人类或动物。
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储存分类代码
12 - Non Combustible Liquids
WGK
WGK 2
闪点(°F)
Not applicable
闪点(°C)
Not applicable
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