AB9298
Anti-MARCKS Antibody, Complete sequence
Chemicon®, from rabbit
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关于此项目
UNSPSC Code:
12352203
NACRES:
NA.41
eCl@ss:
32160702
Conjugate:
unconjugated
Clone:
polyclonal
Application:
western blot
Species reactivity:
rat
Citations:
3
Technique(s):
western blot: suitable
Uniprot accession no.:
产品名称
Anti-MARCKS Antibody, Complete sequence, Chemicon®, from rabbit
biological source
rabbit
conjugate
unconjugated
antibody form
purified immunoglobulin
antibody product type
primary antibodies
clone
polyclonal
species reactivity
rat
manufacturer/tradename
Chemicon®
technique(s)
western blot: suitable
NCBI accession no.
UniProt accession no.
shipped in
dry ice
target post-translational modification
unmodified
Quality Level
Gene Information
human ... MARCKS(4082)
Analysis Note
Control
POSITIVE CONTROL: Neonatal rat brain extract. MARCKS is heat and acid resistant. Heat or acid-treated supernatants from crude tissue or cell homogenates are good sources to analyze MARCKS content by immunoblot.
POSITIVE CONTROL: Neonatal rat brain extract. MARCKS is heat and acid resistant. Heat or acid-treated supernatants from crude tissue or cell homogenates are good sources to analyze MARCKS content by immunoblot.
Application
Anti-MARCKS Antibody, Complete sequence is an antibody against MARCKS for use in WB.
Immunoblotting: 1:1,000 using rat brain extract (colormetric; higher dilutions may be used with ECL). The antibody reacts with the 70-85 kDa (depending on species) MARCKS protein. The suggested dilution buffer is TBS containing 0.05% Tween-20. Suggested blocking buffer is 5% non-fat dry milk. Suggested transfer membrane is PVDF-Immobilon. Suggested gel percentage is 8%. Overnight incubation with the antibody is recommended.
Optimal working dilutions must be determined by the end user.
Optimal working dilutions must be determined by the end user.
Biochem/physiol Actions
Recognizes MARCKS.
Immunogen
Full length recombinant rat MARCKS.
Other Notes
Replaces: AB5427
Physical form
Format: Purified
Semi-purified immunoglobulin. Liquid in 120 mM NaCl, 25 mM Tris-HCl, pH 7.3 containing 0.01% sodium azide.
Legal Information
CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany
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存储类别
10 - Combustible liquids
wgk
WGK 1
Dan Yu et al.
Journal of vascular surgery, 70(6), 2021-2031 (2019-04-02)
Restenosis limits the durability of all cardiovascular reconstructions. Vascular smooth muscle cell (VSMC) proliferation drives this process, but an intact, functional endothelium is necessary for vessel patency. Current strategies to prevent restenosis employ antiproliferative agents that affect both VSMCs and
Dan Yu et al.
PloS one, 10(11), e0141397-e0141397 (2015-11-04)
Overexpression of the myristolated alanine-rich C kinase substrate (MARCKS) occurs in vascular proliferative diseases such as restenosis after bypass surgery. MARCKS knockdown results in arrest of vascular smooth muscle cell (VSMC) proliferation with little effect on endothelial cell (EC) proliferation.
Dan Yu et al.
Journal of the American Heart Association, 4(10), e002255-e002255 (2015-10-10)
Transcription of the myristoylated alanine-rich C kinase substrate (MARCKS) is upregulated in animal models of intimal hyperplasia. MARCKS knockdown inhibits vascular smooth muscle cell (VSMC) migration in vitro; however, the mechanism is as yet unknown. We sought to elucidate the
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