ABC242
Anti-CRKL Antibody
from rabbit, purified by affinity chromatography
别名:
Crk-like protein
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关于此项目
UNSPSC Code:
12352203
NACRES:
NA.41
eCl@ss:
32160702
Conjugate:
unconjugated
Clone:
polyclonal
Application:
IP, WB
Citations:
2
biological source
rabbit
conjugate
unconjugated
antibody form
affinity isolated antibody
antibody product type
primary antibodies
clone
polyclonal
purified by
affinity chromatography
species reactivity
rat, human, mouse
species reactivity (predicted by homology)
rhesus macaque (based on 100% sequence homology)
technique(s)
immunoprecipitation (IP): suitable, western blot: suitable
NCBI accession no.
UniProt accession no.
shipped in
wet ice
target post-translational modification
unmodified
Quality Level
Gene Information
human ... CRKL(1399)
General description
Crk-like protein (CRKL) is a 39 kDa adaptor protein with one SH2 and two SH3 binding domains. CRKL is the major protein which is tyrosine phosphorylated in response to BCR/ABL (chronic myelogenous leukemia) and growth factor activation. In myeloid cells, it binds to proteins such as paxillin, p130cas and p120 cbl. CRKL aslo appears to bind ABL and SOS.
~39 kDa observed. Uncharacterized band(s) may appear in some lysates.
Immunogen
KLH-conjugated linear peptide corresponding to human CRKL.
Application
Detect Crk-like protein using this rabbit polyclonal antibody, Anti-CRKL Antibody validated for use in western blotting & IP.
Research Category
Apoptosis & Cancer
Apoptosis & Cancer
Research Sub Category
Apoptosis - Additional
Apoptosis - Additional
Western Blotting Analysis: 0.25 µg/mL from a representative lot detected CRKL in 10 µg of NIH3T3 and L6 cell lysate.
Immunoprecipitation Analysis: 10 µg from a representative lot immunoprecipitated CRKL in 500 µg of Jurkat cell lysate.
Immunoprecipitation Analysis: 10 µg from a representative lot immunoprecipitated CRKL in 500 µg of Jurkat cell lysate.
Physical form
Affinity purified
Purified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Preparation Note
Stable for 1 year at 2-8°C from date of receipt.
Analysis Note
Evaluated by Western Blotting in Jurkat cell lysate.
Western Blotting Analysis: 0.25 µg/mL of this antibody detected CRKL in 10 µg of Jurkat cell lysate.
Western Blotting Analysis: 0.25 µg/mL of this antibody detected CRKL in 10 µg of Jurkat cell lysate.
Other Notes
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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存储类别
12 - Non Combustible Liquids
wgk
WGK 1
flash_point_f
Not applicable
flash_point_c
Not applicable
Hong Ji et al.
Oncology letters, 12(5), 3811-3817 (2016-11-30)
Overexpression of Crk-like (CrkL) adapter protein has been implicated in a number of types of human cancer. However, its involvement in human cervical carcinoma remains unclear. The present study aimed to explore the clinical significance and biological characteristics of CrkL
Le Cai et al.
Oncology letters, 13(1), 51-56 (2017-01-27)
The v-Crk avian sarcoma virus CT10 oncogene homolog-like (CRKL) protein is important in cancer progression. However, its expression pattern and biological roles in human endometrial carcinoma remain unexplored. The potential mechanism of CRKL-induced cancer progression is still unclear. The present
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