Endoplasmic reticulum resident protein 57 (ERp57) is also called Protein disulfide-isomerase A3 (PDIA3), 58 kDa glucose-regulated protein (GRP58), 58 kDa microsomal protein (p58), Disulfide isomerase ER-60, and Endoplasmic reticulum resident protein 60 (ERp60). ERp57 catalyzes the rearrangement of -S-S- bonds in proteins and is associated with signal transduction, nuclear protein import and apoptosis. ERp57 is expressed in the flagellum and head regions of spermatozoa.

~57 kDa observed

Recombinant protein corresponding to human ERp57.

Chromatin Immunoprecipitation Analysis: A representative lot from an independent laboratory lot detected ERp57 in M14 cell lysates (Chichiarelli, S., et al. (2010). Arch Biochem Biophys. 494(2):178-183; Chichiarelli, S., et al. (2007). J Cell Physiol. 210(2):343-351.).

Western Blotting Analysis: A 1:2,000 dilution from a representative lot detected ERp57 in NPA, WRO, ARO and NCI cell lysates (Prof. F. Altieri, University of Rome, La Sapienza).

Detect ERp57 using this rabbit polyclonal antibody, Anti-ERp57 Antibody validated for use in western blotting & Chromatin IP (ChIP).

Research Category
Epigenetics & Nuclear Function

Research Sub Category
Transcription Factors

Unpurified

Rabbit polyclonal in buffer containing serum with 0.05% sodium azide.

Stable for 1 year at -20°C from date of receipt.
Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.

Evaluated by Western Blotting in HeLa cell lysate.

Western Blotting Analysis: 1:1,000 dilution of this antibody detected ERp57 in 10 µg of HeLa cell lysate.

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

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