跳转至内容
Merck
CN
登录查看公司和协议定价

About This Item

UNSPSC代码:
12352203
eCl@ss:
32160702
技术服务
需要帮助?我们经验丰富的科学家团队随时乐意为您服务。
让我们为您提供帮助

生物来源

rabbit

质量水平

偶联物

unconjugated

抗体形式

affinity isolated antibody

抗体产品类型

primary antibodies

克隆

polyclonal

纯化方式

affinity chromatography

种属反应性

human

技术

western blot: suitable

NCBI登记号

UniProt登记号

运输

ambient

靶向翻译后修饰

unmodified

基因信息

human ... FANCI(55215)

一般描述

Fanconi anemia group I protein (UniProt Q9NVI1; also known as Protein FACI) is encoded by the FANCI (also known as KIAA1794) gene (Gene ID 55215) in human. It plays a vital role in the repair of DNA double-strand breaks by homologous recombination and in the repair of interstrand DNA cross-links (ICLs) by promoting FANCD2 monoubiquitination by FANCL and participating in recruitment to DNA repair sites. It is also required for maintenance of chromosomal stability. FACI is reported to participate in S phase and G2 phase checkpoint activation upon DNA damage. It is monoubiquitinated by FANCL on Lys-523 during S phase and upon genotoxic stress and is deubiquitinated by USP1 as cells enter G2/M or once the DNA repair is completed. Mutations in the FANCI gene are known to cause Fanconi anemia, a developmental and cancer-predisposition syndrome. Ref: Smogorzewska A., et al. (2007) Cell 129, 289-301.

特异性

This polyclonal antibody detected the expected target band only in lysate from FANCI-tranfected, but not untransfected FANCI-deficient EUFA1301 cells.

免疫原

Synthetic peptide corresponding to an internal sequence within the N-terminal half of human FANCI.

应用

Anti-FANCI (G4270), Cat. No. ABE1817, is a highly specific rabbit polyclonal antibody that targets FANCI and has been tested in Western Blotting.
Research Category
Epigenetics & Nuclear Function
Western Blotting Analysis: 1.4 µg/mL from a representative lot detected exogenously expressed FANCI in transfected, but not untransfected FANCI-deficient EUFA1301 cells (kindly provided by Dr. Johan de Winter) with or without 24-hour 60 ng/ml mitomycin C (MMC) treatment (Courtesy of Martin Cohn, Ph.D., Department of Biochemistry, University of Oxford, UK).

Western Blotting Analysis: 1.4 µg/mL from a representative lot detected endogenous FANCI in FANCD2-deficient EUFA1421 (kindly provided by Dr. Johan de Winter) cells. Exogenous FANCD2 expression stabilized FANCI and monoubiquitinated FANCI was detectable upoon 24-hour 60 ng/ml mitomycin C (MMC) treatment of FANCD2 transfectants (Courtesy of Martin Cohn, Ph.D., Department of Biochemistry, University of Oxford, UK).

Western Blotting Analysis: 1.4 µg/mL from a representative lot detected monoubiquitinated FANCI mainly in the nuclear fraction of FANCD2-deficient EUFA1421 cells (kindly provided by Dr. Johan de Winter) upon exogenous FANCD2 expression with or without additional 24-hour 60 ng/ml mitomycin C (MMC) treatment (Courtesy of Martin Cohn, Ph.D., Department of Biochemistry, University of Oxford, UK).

质量

Evaluated by Western Blotting in K562 nuclear extract.

Western Blotting Analysis: 0.5 µg/mL of this antibody detected FANCI in 10 µg of K562 nuclear extract.

目标描述

~160 kDa observed. 149.3 kDa calculated. Uncharacterized bands may be observed in some lysate(s).

外形

Affinity purified.
Purified rabbit polyclonal antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

储存及稳定性

Stable for 1 year at 2-8°C from date of receipt.

其他说明

Concentration: Please refer to lot specific datasheet.

免责声明

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

未找到合适的产品?  

试试我们的产品选型工具.

储存分类代码

12 - Non Combustible Liquids

WGK

WGK 1

闪点(°F)

Not applicable

闪点(°C)

Not applicable


分析证书(COA)

输入产品批号来搜索 分析证书(COA) 。批号可以在产品标签上"批“ (Lot或Batch)字后找到。

已有该产品?

在文件库中查找您最近购买产品的文档。

访问文档库

我们的科学家团队拥有各种研究领域经验,包括生命科学、材料科学、化学合成、色谱、分析及许多其他领域.

联系客户支持