Putative adenosylhomocysteinase 2 (EC 3.3.1.1; UniProt O43865; also known as AdoHcyase 2, DC-expressed AHCY-like molecule, IP(3)Rs binding protein released with IP(3), IRBIT, S-adenosyl-L-homocysteine hydrolase 2, S-adenosylhomocysteine hydrolase-like protein 1) is encoded by the AHCYL1 (also known as DCAL, PPP1R78, PRO0233, XPVKONA) gene (Gene ID 10768) in human. Originally identified by its binding and antagonist activity against teh intracellular calcium channel, IP3R, IRBIT is now known to interact with multiple targets and regulate diverse physiological events in addition to blocking IP3 access to IP3R. IRBIT complexes with ribonucleotide reductase (RNR) in a dATP-dependent manner and stabilizes RNR in its inactive, dATP-bound state. IRBIT also targets CaMKIIα kinase for inhibition by blocking calmodulin binding. Other known IRBIT targets include the ion transporter NBCe1-B, the Na+/H+ exchanger NHE3, the Cl- channel CFTR, the Cl−/HCO3− exchanger Slc26a6, the Fip1 component of the cleavage and polyadenylation specificity factor (CPSF) complex. IRBIT contains an S-adenosyl-l-homocysteine hydrolase (AHCY) homology domain in and an N-terminal extension containing a serine-rich region with multiple phosphorylation sites. Long-IRBIT, but not IRBIT, has an additional N-terminal appendage, termed the LISN (Long-IRBIT specific N-terminal) domain. IRBIT is highly conserved among species, and the human and mouse orthologs have 100% protein identity.

~70 kDa observed. 59.0 kDa (isoform 1) and 53.8 kDa (isoform 2) calculated. Uncharacterized band(s) may appear in some lysates.

Recombinant protein corresponding to human AHCYL1(IRBIT).

Research Category
Epigenetics & Nuclear Function

Research Sub Category
Nuclear Receptors

Western Blotting Analysis: A representative lot detected Tet-inducible short hairpin RNA-mediated AHCYL1 (IRBIT) downregulation in HeLa cells (Arnaoutov, A., and Dasso, M. (2014). Science 345(6203):1512-1515).
Western Blotting Analysis: A representative lot detected immunoprecipitated AHCYL1 (IRBIT) from HeLa lysates by Western blotting (Arnaoutov, A., and Dasso, M. (2014). Science 345(6203):1512-1515).
Western Blotting Analysis: A representative lot detected the association of His-tagged human AHCYL1 (IRBIT) with human IP3R1(1-604) GST fusion protein by Western blotting analysis of the affinity pull-down obtained with Glutathione beads (Arnaoutov, A., and Dasso, M. (2014). Science 345(6203):1512-1515).

Expected to react with both spliced isoforms reported by UniProt (O43865-1 and O43865-2).

Affinity purified

Purified chicken polyclonal antibody in PBS with 0.02% Sodium azide.

Stable for 1 year at 2-8°C from date of receipt.

Evaluated by Western Blotting in HeLa cell lysate.

Western Blotting Analysis: A 1:500 dilution of this antibody detected AHCYL1 (IRBIT) in 10 µg of HeLa cell lysate.

Concentration: Please refer to lot specific datasheet.

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