Anti-HEXIM1 Antibody

HEXIM1 is a transcriptional regulator that functions as a general RNA polymerase II transcription inhibitor. HEXIM1 works in cooperation with 7SK snRNA to sequester P-TEFb in a large inactive 7SK snRNP complex, preventing RNA polymerase II phosphorylation and subsequent transcriptional elongation. The 7SK snRNP complex is composed of P-TEFb (composed of CDK9 and CCNT1/cyclin-T1), HEXIM1, HEXIM2, BCDIN3, SART3 proteins and 7SK and U6 snRNAs. Hexim1 also interacts with the N-CoR complex through NCOR1, ESR1, NR3C1, and may interact with NF-kappa-B through REL A. HEXIM1 is located in the nucleus where it can bind with alpha-importin, and can also be found in the cytoplasm. It is up-regulated by HMBA (hexamethylene bisacetamide) at the protein level and down-regulated by estrogen.

~52 kDa observed. An uncharacterized band at ~60 kDa has been observed in some lysates.

Recombinant protein corresponding to human HEXIM1.

Immunocytochemistry Analysis: 1:500 dilution from a representative lot detected HEXIM1 in HeLa and A431 cells.

Research Category
Epigenetics & Nuclear Function

Epigenetics & Nuclear Function

Research Sub Category
Transcription Factors

Chromatin Biology

Use Anti-HEXIM1 Antibody (rabbit polyclonal antibody) validated in WB, ICC to detect HEXIM1 also known as Cardiac lineage protein 1, Estrogen down-regulated gene 1 protein ,Hexamethylene bis-acetamide-inducible protein 1.

Rabbit polyclonal serum containing 0.05% sodium azide.

Unpurified

Stable for 1 year at -20°C from date of receipt.
Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.

Control
HeLa cell lysate

Evaluated by Western Blot in HeLa cell lysate.

Western Blot Analysis: 1:1,000 dilution of this antibody detected HEXIM1 on 10 µg of HeLa cell lysate.

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