ABE278
Anti-MILI Antibody
from rabbit, purified by affinity chromatography
别名:
Piwi-like protein 2
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关于此项目
UNSPSC Code:
12352203
NACRES:
NA.41
eCl@ss:
32160702
Conjugate:
unconjugated
Clone:
polyclonal
Application:
immunocytochemistry
immunohistochemistry
immunoprecipitation (IP)
western blot
immunohistochemistry
immunoprecipitation (IP)
western blot
Species reactivity:
rat, mouse
Citations:
Technique(s):
immunocytochemistry: suitable
immunohistochemistry: suitable
immunoprecipitation (IP): suitable
western blot: suitable
immunohistochemistry: suitable
immunoprecipitation (IP): suitable
western blot: suitable
Uniprot accession no.:
产品名称
Anti-MILI Antibody, from rabbit, purified by affinity chromatography
biological source
rabbit
conjugate
unconjugated
antibody form
affinity isolated antibody
antibody product type
primary antibodies
clone
polyclonal
purified by
affinity chromatography
species reactivity
rat, mouse
technique(s)
immunocytochemistry: suitable
immunohistochemistry: suitable
immunoprecipitation (IP): suitable
western blot: suitable
NCBI accession no.
UniProt accession no.
shipped in
wet ice
target post-translational modification
unmodified
Quality Level
Gene Information
mouse ... Piwil2(57746)
rat ... Piwil2(306011)
Analysis Note
Control
RAW264.7 cell lysate
RAW264.7 cell lysate
Evaluated by Western Blot in RAW264.7 cell lysate.
Western Blot Analysis: A 1:1,000 dilution of this antibody detected MILI in 10 µg of RAW264.7 cell lysate.
Western Blot Analysis: A 1:1,000 dilution of this antibody detected MILI in 10 µg of RAW264.7 cell lysate.
Application
Anti-MILI Antibody is a Rabbit Polyclonal Antibody for detection of MILI also known as Piwi-like protein 2 & has been validated in WB, ICC, IHC & IP.
Immunocytochemistry Analysis: A representative lot was used by an independent laboratory in male primordial germ cells. (Aravin, A.A., et al. (2008). Mol Cell. 31(6):785-799.)
Immunohistochemistry Fluoresence Analysis: A representative lot was used by an independent laboratory in embyronic testis cross sections. (Vagin, W., et al. (2009). Genes Dev. 23(15):1749-1762.)
Immunoprecipitation Analysis: A representative lot was used by an independent laboratory. (Aravin, A.A., et al. (2007). Science. 316(5825):744-747.)
Immunohistochemistry Fluoresence Analysis: A representative lot was used by an independent laboratory in embyronic testis cross sections. (Vagin, W., et al. (2009). Genes Dev. 23(15):1749-1762.)
Immunoprecipitation Analysis: A representative lot was used by an independent laboratory. (Aravin, A.A., et al. (2007). Science. 316(5825):744-747.)
Research Category
Epigenetics & Nuclear Function
Epigenetics & Nuclear Function
Research Sub Category
Cell Cycle, DNA Replication & Repair
Cell Cycle, DNA Replication & Repair
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
General description
MILI, MIWI, and MIWI2 belong to the Piwi family of proteins. piRNAs and Piwi proteins are implicated in transposon control and are linked to transposon methylation in mammals. MILI, in particular, directly binds with piRNAs in the cytoplasm and is required for PIWIL4/MIWI2 nuclear localization and association with secondary piRNAs antisense. MILI plays an essential role in spermatogenesis, meiotic differentiation of spermatocytes, germ cell differentiation and in self-renewal of spermatogonial stem cells.
~109 kDa observed. An uncharacterized bands at ~30, 55, and 60 kDa may be observed in some lysates.
Immunogen
KLH-conjugated linear peptide corresponding to mouse MILI.
Physical form
Affinity purified
Purified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Preparation Note
Stable for 1 year at 2-8°C from date of receipt.
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存储类别
12 - Non Combustible Liquids
wgk
WGK 2
flash_point_f
Not applicable
flash_point_c
Not applicable
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