Anti-dimethyl Histone H4 (Arg3), Asymmetric Antibody

Histones are highly conserved proteins that serve as the structural scaffold for the organization of nuclear DNA into chromatin. The histones have an amino terminal tail, a globular domain, and a carboxy-terminal tail. The four core histones, H2A, H2B, H3 and H4 assemble into the octamer (2 molecules of each). Subsequently, 146 base pairs of DNA are wrapped in a sequence-independent manner around the octamer, forming the basic subunit of chromatin, the nucleosome. Histone H4 is one of the 5 main histone proteins involved in the structure of chromatin in eukaryotic cells. Featuring a main globular domain and a long N terminal tail H4 is involved with the structure of the nucleosomes of the ′beads on a string′ structure.

~13 kDa observed

Epitope: N-terminus

KLH-conjugated linear peptide corresponding to the N-terminus of human Histone H4, asymmetrically dimethylated at Arg3.

Immunocytochemistry Analysis: A 1:100 dilution from a representative lot detected dimethyl Histone H4 (Arg3), Asymmetric in HeLa cells.

Dot Blot (Specificity) Analysis: A 1:10,000 dilution from a representative lot detected dimethyl Histone H4 (Arg3), Asymmetric in an Absurance Histone H3 Antibody Specificity Array (Cat. No. 16-667) and an Absurance Histone H2A, H2B, H4 Antibody Specificity Array (Cat. No. 16-665).

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Research Category
Epigenetics & Nuclear Function

Research Sub Category
Histones

This Anti-dimethyl Histone H4 (Arg3) antibody, Asymmetric is validated for use in western blotting, ICC & dot blot for the detection of dimethyl Histone H4 (Arg3).

Affinity purified

Purified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Stable for 1 year at 2-8°C from date of receipt.

Evaluated by Western Blotting in untreated and CARM1 treated recombinant Histone H4.

Western Blotting Analysis: A 1:50,000 dilution of this antibody detected dimethyl Histone H4 (Arg3), Asymmetric in 0.5 µg of CARM1 treated recombinant Histone H4.

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