Anti-hMOF Antibody

hMOF, also known as Histone acetyltransferase KAT8, or Lysine acetyltransferase, MOZ, YBF2/SAS3, SAS2, TIP60 protein 1, and MYST-1, and encoded by the gene KAT8/MOF/MYST1, is a histone acetyltransferase which is involved in transcriptional activation and also is a component of the MSL complex involved in acetylation of nucleosomal histone H4 producing specifically H4K16ac. As part of the NSL complex it may be involved in acetylation of nucleosomal histone H4 on several lysine residues. hMOF also a component of many other MSL related complexes including the methyltransferase complexes and interacts with numerous other related proteins including BAP18, CHD8, E2F6, HSP70, INO80C, KANSL1, LAS1L, MAX, MCRS1, MGA, MOF/KAT8, PELP1, PHF20, PRP31, RING2, RUVB1/TIP49A, RUVB2/TIP49B, SENP3, TAF1, TAF4, TAF6, TAF7, TAF9 and TEX10. hMOF is localized to the nucleus and nuclear chromosomes.

~60 kDa observed. Uncharacterized band(s) may be observed in some cell lysate.

Linear peptide corresponding to hMOF.

Anti-hMOF antibody detects level of hMOF & has been published & validated for use in hMOF.

Chromatin Immunoprecipitation Analysis: A representative lot from an independent laboratory immunoprecipitated hMOF from HeLa cell lysate (Wu, L., et al. (2011). Mol Cell. 43(1):132-144.).

Immunoprecipitation Analysis: A representative lot from an independent laboratory immunoprecipitated hMOF from HeLa cell lysate (Smith, E. R., et al. (2005). Mol Cell Biol. 25(21):9175-9188.).

Research Category
Epigenetics & Nuclear Function

Research Sub Category
Chromatin Biology

Format: Purified

Protein G Purified

Purified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Stable for 1 year at 2-8°C from date of receipt.

Evaluated by Western Blotting in HUVEC cell lysate.

Western Blotting Analysis: 1 µg/mL of this antibody detected hMOF in 10 µg of HUVEC cell lysate.

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

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