biological source
rabbit
antibody form
serum
antibody product type
primary antibodies
clone
polyclonal
species reactivity
mouse, rat, human
technique(s)
immunocytochemistry: suitable, immunohistochemistry: suitable, immunoprecipitation (IP): suitable, western blot: suitable
NCBI accession no.
UniProt accession no.
shipped in
wet ice
target post-translational modification
unmodified
Gene Information
human ... CETN3(1070)
General description
Centrin-3 is critical to microtubule-organizing center structure and function. Centrin-3 has been linked to cell cycle, cell division and mitotic processes and is localized in the centrosome of interphase and mitotic cells.
~19 kDa observed. Uncharacterized band(s) may be observed in some cell lysates.
Immunogen
Recombinant protein corresponding to human Centrin-3.
Application
Detect Centrin using this rabbit polyclonal antibody, Anti-Centrin-3 Antibody validated for use in western blotting, IP, ICC & IHC.
Immunoprecipitation Analysis: A representative lot from an independent laboratory immunoprecipitated Centrin-3 in MDCK cell lysates (Koch, A., et al. (2010). Mol Biol Cell. 21(2):219-231.).
Immunohistochemistry Analysis: A representative lot from an independent laboratory detected Centrin-3 in MDCK cell lysates (Koch, A., et al. (2010). Mol Biol Cell. 21(2):219-231.).
Immunocytochemistry Analysis: A representative lot from an independent laboratory detected Centrin-3 in HeLa cells (Klein, U. R., ad Nigg, E. A. (2009). J Cell Sci. 12(Pt18):3312-321.).
Immunohistochemistry Analysis: A representative lot from an independent laboratory detected Centrin-3 in MDCK cell lysates (Koch, A., et al. (2010). Mol Biol Cell. 21(2):219-231.).
Immunocytochemistry Analysis: A representative lot from an independent laboratory detected Centrin-3 in HeLa cells (Klein, U. R., ad Nigg, E. A. (2009). J Cell Sci. 12(Pt18):3312-321.).
Research Category
Epigenetics & Nuclear Function
Epigenetics & Nuclear Function
Research Sub Category
Chromatin Biology
Chromatin Biology
Physical form
Rabbit polyclonal serum with 0.05% sodium azide.
Unpurified
Preparation Note
Stable for 1 year at -20°C from date of receipt.
Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
Analysis Note
Control
RAW264.7 cell lysate
RAW264.7 cell lysate
Evaluated by Western Blotting in RAW264.7 cell lysate.
Western Blotting Analysis: A 1:1,000 dilution from a representative lot detected Centrin-3 in 10 µg of RAW264.7 cell lysate.
Western Blotting Analysis: A 1:1,000 dilution from a representative lot detected Centrin-3 in 10 µg of RAW264.7 cell lysate.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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存储类别
12 - Non Combustible Liquids
wgk
WGK 1
flash_point_f
Not applicable
flash_point_c
Not applicable
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