Anti-Centrin-3 Antibody

Centrin-3 is critical to microtubule-organizing center structure and function. Centrin-3 has been linked to cell cycle, cell division and mitotic processes and is localized in the centrosome of interphase and mitotic cells.

~19 kDa observed. Uncharacterized band(s) may be observed in some cell lysates.

Recombinant protein corresponding to human Centrin-3.

Detect Centrin using this rabbit polyclonal antibody, Anti-Centrin-3 Antibody validated for use in western blotting, IP, ICC & IHC.

Immunoprecipitation Analysis: A representative lot from an independent laboratory immunoprecipitated Centrin-3 in MDCK cell lysates (Koch, A., et al. (2010). Mol Biol Cell. 21(2):219-231.).

Immunohistochemistry Analysis: A representative lot from an independent laboratory detected Centrin-3 in MDCK cell lysates (Koch, A., et al. (2010). Mol Biol Cell. 21(2):219-231.).

Immunocytochemistry Analysis: A representative lot from an independent laboratory detected Centrin-3 in HeLa cells (Klein, U. R., ad Nigg, E. A. (2009). J Cell Sci. 12(Pt18):3312-321.).

Research Category
Epigenetics & Nuclear Function

Research Sub Category
Chromatin Biology

Unpurified

Rabbit polyclonal serum with 0.05% sodium azide.

Stable for 1 year at -20°C from date of receipt.
Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.

Control
RAW264.7 cell lysate

Evaluated by Western Blotting in RAW264.7 cell lysate.

Western Blotting Analysis: A 1:1,000 dilution from a representative lot detected Centrin-3 in 10 µg of RAW264.7 cell lysate.

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.