Anti-WBP-7 Antibody

WW domain-binding protein 7 (WBP-7) is also called Histone-lysine N-methyltransferase MLL4, Lysine N-methyltransferase 2B (KMT2B), Myeloid/lymphoid or mixed-lineage leukemia protein 4 and Trithorax homolog 2. WBP-7 is a histone methyltransferase that methylates ′Lys-4′ of histone H3, a specific tag for epigenetic transcriptional activation. WBP-7 is important in beta-globin locus transcription regulation and for controlling bulk H3K4 trimethylation (H3K4me3), global transcriptional silencing, oocyte survival and normal zygotic genome activation during oocyte growth and development. WBP-7 is widely expressed with the highest levels in testis. WBP-7 is also detected in brain, bone marrow, heart, muscle, kidney, placenta, spleen, thymus, prostate, ovary, intestine, colon, peripheral blood lymphocytes and pancreas and is found to often be amplified in pancreatic carcinomas.

~75 kDa observed. The full length form and the cleaved form of WBP-7 may be observed at 300 kDa and 75 kDa, respectively (Natarajam, T. G., et al. (2010). Cancer Cell Int. 10:13).

Epitope: Near C-Terminus

KLH-conjugated linear peptide corresponding to human WBP-7 near the C-Terminus.

Detect WW domain-binding protein 7 using this rabbit polyclonal antibody, Anti-WBP-7 Antibody validated for use in western blotting.

Research Category
Epigenetics & Nuclear Function

Research Sub Category
Chromatin Biology

This antibody recognizes WBP-7 near the C-Terminus.

Affinity purified

Purified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Stable for 1 year at 2-8°C from date of receipt.

Evaluated by Western Blotting in MCF7 cell lysate.

Western Blotting Analysis: 2.0 µg/mL of this antibody detected MCF7 in 10 µg of MCF7 cell lysate.

Control
MCF7 cell lysate

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

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