Anti-Ubiquitin Histone H2A (Lys119) Antibody

Histones are highly conserved proteins that serve as the structural scaffold for the organization of nuclear DNA into chromatin. Histones are modified post-translationally by acetylation, phosphorylation, methylation, and ubiquitination and these modifications regulate DNA transcription, repair, recombination, and replication. Ubiquitylation usually targets the substrate for degradation, although histones H2A and H2B are actually stabilized by a single ubiquitin conjugation. Histone ubiquitination has been correlated with DNA repair and transcription, cellular differentiation, cell cycle regulation, spermatogenesis, protein trafficking, and response to stress. Histone H2A is monoubiquitinated at Lys119 by the PRC-1L complex (Polycomb repressive complex 1-like), which includes Ring1, Ring2, Bmi1 and HPH2. Ubiquitinated H2A normally represents about 15% of H2A, but this value can be as high as 50% in active chromatin. Ubiquitinyl histone H2A has also been associated with transcriptional silencing of large chromatin regions and linked to Polycomb silencing so the function of ubiquitinated H2A remains undefined.

~23 kDa observed

Epitope: Near C-terminus

KLH-conjugated linear peptide corresponding to human Ubiquitin histone H2A (Lys119) near the C-terminus.

Immunoprecipitation Analysis: 10ug from a representative lot immunoprecipitated Ubiquitin histone H2A (Lys119) from 0.5 mgs of HeLa cell lysate.
Dot Blot Analysis: A 1:20,000 dilution from a representative lot detected Ubiquitin histone H2A (Lys119) in peptides with various modifications.

Research Category
Epigenetics & Nuclear Function

Research Sub Category
Nuclear Receptors

This Anti-Ubiquitin Histone H2A (Lys119) Antibody is validated for use in Western Blotting and Dot Blot and Immunoprecipitation for the detection of Ubiquitin Histone H2A (Lys119).

Affinity purified

Purified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Stable for 1 year at 2-8°C from date of receipt.

Evaluated by Western Blotting in HeLa cell lysate.

Western Blotting Analysis: 2.0 µg/mL of this antibody detected Ubiquitin histone H2A (Lys119) in 10 µg of HeLa cell lysate.

Concentration: Please refer to lot specific datasheet.

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