Anti-MCM3 Antibody Anitbody

MCM3 (minichromosome maintenance complex component 3) is part of the hexameric MCM DNA helicase, a complex replicative helicase, which plays essential roles in DNA replication.

~ 105 kDa observed

KLH-conjugated linear peptide corresponding to human MCM3.

Detect MCM3 using this rabbit polyclonal antibody, Anti-MCM3 Antibody Anitbody validated for use in western blotting & ICC.

Research Category
Epigenetics & Nuclear Function

Research Sub Category
Transcription Factors

Western Blotting Analysis: A representative lot detected MCM3 in IMR90 cell lysate (Chicas, A., et al. (2012). PNAS. 109:(23)8971-8976).
Western Blotting Analysis: A representative lot detected MCM3 in asynchronous culture of Raji cell lysates (Mendes, J., et al. (2000). Molecular and Cellular Biology. 20:(22)8602-8612).
Western Blotting Analysis: A representative lot detected MCM3 in KB cells, derived from human nasopharyngeal epidermoid carcinoma and breast cancer-derived cell lines. (Ekholm-Reed, S., et al. (2004). Journal of Cell Biology. 165(6):789-800).
Immunofluorescence Analysis: A representative lot detected MCM3 in HeLa and MCF-7 cell lysate (Prasanth, et al. (2004). Philosophical Transactions: Biological Sciences. 359:153-154).
Immunofluorescence Analysis: A representative lot detected MCM3 in in KB cells, derived from human nasopharyngeal epidermoid carcinoma and breast cancer-derived cell lines (Ekholm-Reed, S., et al. (2004). Journal of Cell Biology. 165(6):789-800).

Rabbit polyclonal in buffer containing serum with 0.05% sodium azide.

Unpurified

Stable for 1 year at -20°C from date of receipt.
Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.

Evaluated by Western Blotting in HEK293T cell lysate.

Western Blotting Analysis: A 1:1,000 dilution of this antibody detected MCM3 in 10 µg of HEK293T cell lysate.

Replaces: MABE544

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