Anti-Adenovirus 5 packaging protein 52K

Packaging protein 3 (UniProt: P04496; also known as L1-52/55 kDa protein, Packaging protein 52K) is encoded by the L1 gene (Gene ID: 2652993) in human Adenovirus 5. Adenovirus 5 belongs to the Adenoviridae family of non-enveloped viruses that are characterized by linear, double-stranded DNA genomes. Their capsid is an icosahedron that contains at least 12 different viral proteins. Adenovirus 5 is commonly used for gene transfer experiments due to its ability to infect a wide group of different cell types. It can harbor large genes in its genome incorporated via homologous recombination techniques. The 52 kDa packaging protein of human adenovirus 5 is part of the genome packaging machinery that consists of polypeptides IIIa, IVa2, L4 33k, and L4 22k. It is expressed in the early phase and late phase of viral replicative cycle. Its role during assembly consists in tethering the viral core to the icosahedral shell wherein maturation proceeds simultaneously with packaging, before the viral particle is sealed. Its adenovirus protease consensus cleavage site is localized at amino acids 351-352. It plays a critical role in the coordinated assembly of infectious progeny particles and contributes to the specificity of packaging, possibly by interacting with capsid protein IIIa. It is proteolytically cleaved at different sites during virion maturation. It is reported to be present in empty particles in its full-length form but is absent from the mature virion. (Ref.: Charman, M., et al. (2023). Nature. 616(7956):332-338; Pérez-Berná, AJ., et al. (2014). J. Virol. 88(3); 1513-1524).

GST-tagged full-length, recombinant packaging protein 52K from human Adenovirus 5.

Quality Control Testing

Evaluated by Western Blotting in lysate from Human adenovirus type 5 infected HeLa cells.

Western Blotting Analysis: A 1:1,000 dilution of this antibody detected packaging protein 52K from human adenovirus 5 in lysate from HeLa cells infected with Human adenovirus type 5, but not in lysate from uninfected HeLa cells.

Tested Applications

Immunofluorescence Analysis: A representative lot detected Adenovirus packaging protein 52K in immunofluorescence application. ( Charman, M., et al. (2023). Nature. 616(7956):332-3380).

Western Blotting Analysis: A 1:2,500 dilution from a representative lot detected Adenovirus packaging protein 52K in lysates from HeLa cells infected with Human adenovirus type 5. (Courtesy of Dr. Patrick Hearing, Stony Brook University).

Western Blotting Analysis: A representative lot detected Adenovirus packaging protein 52K in Western Blotting application. ( Subramanian, T., et al. (2006). J. Virol. 80(4); 2000-2012).

Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user.

This rabbit polyclonal antibody detects Packaging protein 52K of Human Adenovirus 5.

Rabbit polyclonal antisera without azide.

Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.

Store at -10°C to -25°C. Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

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