Anti-BAALC

Western Blotting Analysis: A 1:500 dilution from a representative lot detected Brain and acute leukemia cytoplasmic protein (BAALC) in mouse brain tissue lysate.

Immunohistochemistry (Paraffin) Analysis: A 1:250 dilution from a representative lot detected Brain and acute leukemia cytoplasmic protein (BAALC) in human cerebral cortex and rat hippocampus tissue sections.

Western Blotting Immunohistochemistry (Paraffin)

Anti-BAALC Antibody, Cat. No. ABN2243, is a rabbit polyclonal antibody that detects Brain and acute leukemia cytoplasmic protein (BAALC) and has been tested for use in Immunohistochemistry (Paraffin) and Western Blotting.

This rabbit polyclonal antibody that detects Brain and acute leukemia cytoplasmic protein (BAALC). It targets an epitope within 16 amino acids from the N-terminal region.

KLH-conjugated linear peptide corresponding to 16 amino acids from the N-terminal region of human Brain and acute leukemia cytoplasmic protein (BAALC).

Stable for 1 year at 2-8°C from date of receipt.

Purified rabbit polyclonal antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Brain and acute leukemia cytoplasmic protein (UniProt: Q8WXS3; also known as BAALC) is encoded by the BAALC gene (Gene ID: 79870) in human. BAALC is predominantly expressed in neuroectoderm-derived tissues. Its expression has been reported in the brain and spinal cord, and at low levels, in the adrenal gland. In the bone marrow, it is shown to be confined to the CD34+ progenitor cells. In neurons, it localizes to postsynaptic lipid rafts and in myocardial and skeletal muscle cells, it is shown to be localized to the cytoplasm adjacent to the inner cell membrane. Overexpression of BAALC has been reported in subjects with acute myeloid leukemia (AML) and acute lymphoblastic leukemia (ALL). Its high expression is considered as an independent marker of poor prognosis. In leukemia cells, BAALC induces cell-cycle progression by sustaining ERK activity through an interaction with a scaffold protein MEK kinase-1 that blocks the interaction between ERK and MAP kinase phosphatase 3. It is shown to confer chemoresistance in AML cells by upregulating ATP-binding cassette proteins in an ERK-dependent manner and blocks ERK-mediated monocytic differentiation of AML cells by trapping KLF4 in the cytoplasm and inhibiting its function in the nucleus. (Ref.: Tanner, SM., et al. (2001). Proc. Natl. Acad. Sci. USA. 98(24); 13901-13906; Morita, K., et al. (2015). Leukemia. 29(11); 2248-56).

Evaluated by Western Blotting in human brain tissue lysate.

Western Blotting Analysis: A 1:500 dilution of this antibody detected Brain and acute leukemia cytoplasmic protein (BAALC) in human brain tissue lysate.

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.