Anti-Netrin-1 Antibody

Netrin-1 is a secreted, bifunctional ligand belonging to the Netrin family; a group of proteins known to act as versatile, chemotropic axon guidance cues for CNS commissural and peripheral motor axons. Netrin-1 is necessary for spinal cord oligodendrocyte development. There are two receptors with which Netrin-1 is associated and that will cause it to be either a chemoattractant or a chemorepellant for axons. Receptor Deleted in Colorectal Cancer (DCC) regulates the attraction of axons to Netrin-1, and UNC5 mediates axon repulsion from Netrin-1. Studies have shown Netrin-1 to function as a survival factor through its relationship to its receptors, inhibiting the onset of apoptosis. Netrin-1 has also been implicated in colorectal tumorigenesis via its apoptotic regulatory role. Expression of Netrin-1 has been detected in adult axons and oligodendrocytes, and is also widely observed in developing embryonic mesodermal tissues and nervous system.

~68 kDa observed. Characteristic proteolytic fragments of Netrin-1 found in the CNS run at lower molecular weights.

Epitope: Unknown

Linear peptide corresponding to mouse Netrin-1.

Anti-Netrin-1 Antibody detects level of Netrin-1 & has been published & validated for use in WB.

Research Category
Neuroscience

Research Sub Category
Growth Cones & Axon Guidance

Other homologies: Human (94% sequence homology).

Unpurified

Rabbit polyclonal serum containing 0.05% sodium azide.

Stable for 1 year at -20°C from date of receipt.
Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.

Control
Mouse brain tissue lysate

Evaluated by Western Blot in mouse brain tissue lysate.

Western Blot Analysis: 1:10,000 dilution of this antibody detected Netrin-1 on 10 µg of mouse brain tissue lysate.

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