Anti-phospho-NR1 (Ser896) Antibody

NR1 (NMDA1or NMDAR1, also known as GRIN1) along with the NR2 subunit forms a heterodimer that make up the NMDA receptor. The NMDA receptor (NMDAR) is characterized as a ionotropic receptor for glutamate. As the name suggests, (NMDA (N-methyl D-aspartate) is an agonist for the receptor. A distinct characteristic of the NMDA receptor is that it is both ligand-gated and voltage-dependent based on its sensitivity to magnesium. Activated NMDA receptors result in the opening of an ion channel that is nonselective to cations. The result is the flow of Na+ and small amounts of Ca2+ ions into the cell and K+ out of the cell. Calcium flux by the NMDARs has been implicated in synaptic plasticity, a mechanism in the cell for learning and memory.

~130kDa observed

Epitope: Phosphorylated Ser896

KLH-conjugated linear peptide corresponding to rat NR1 phosphorylated at Ser896.

Detect phospho-NR1 (Ser896) using this Anti-phospho-NR1 (Ser896) Antibody validated for use in WB, IH(P).

Immunohistochemistry Analysis: 1:25 dilution from a previous lot detected NR1 in normal rat hippocampus tissue.

This antibody recognizes NR1 when phosphorylated at Ser896.

Control
Lambda phosphatase untreated and treated rat microsomal brain tissue lysates

Evaluated by Western Blot in lambda phosphatase untreated and treated rat microsomal brain tissue lysates.

Western Blot Analysis: 1 µg/mL of this antibody detected NR1 on 10 µg of lambda phosphatase untreated and treated rat microsomal brain tissue lysates.

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Replaces: 06-640