Anti-citrullinated RGG

Anti-citrullinated RGG, Cat. No. ABS2244, is a rabbit polyclonal antibody that detects citrullinated RGG motifs in proteins and has been tested for use in ELISA and Western Blotting.

ELISA Western Blotting

ELISA Analysis: A representative lot detected citrullinated RGG in ELISA applications (Tanikawa, C., et. al. (2018). Cell Rep. 22(6):1473-1483).

Western Blotting Analysis: A representative lot detected citrullinated RGG in Western Blotting applications (Tanikawa, C., et. al. (2018). Cell Rep. 22(6):1473-1483).

This rabbit polyclonal antibody specifically detects citrullinated RGG motifs in proteins.

A chemically synthesized citrullinated peptide (CGGCitGGGCitGG).

Stable for 1 year at 2-8°C from date of receipt.

Purified rabbit polyclonal antibody in PBS with 0.1% sodium azide.

Evaluated by Western Blotting in HEK293T cells expressing WT PAD4 & mutant PAD4.

Western Blotting Analysis: A 1:1,000 dilution of this antibody detected citrullinated RGG in lysates from HEK293T cells expressing WT PAD4, but not in cells expressing mutant PAD4. RGG citrullination is observed in multiple proteins.

Protein citrullination, also known as deamination, is one of the post-translational modifications, which converts arginine into citrulline residues, which results in loss of a positive charge in the protein and affects protein structure and immunogenicity. The process of citrullination is catalyzed by a calcium-dependent peptidylarginine deiminiases (PADs), which are conserved in all vertebrates. All PADs, with the exception of PAD4, are localized in the cytoplasm. PAD4 is located in the nucleus. Citrullination has been reported on histone proteins, myelin basic protein (MBP) and filaggrin in normal physiological processes and on fibrin and vimentin during inflammation and cell death. RG/RGG is shown to be a consensus sequence for PAD4-mediated citrullination and RG/RGG motifs are of frequent occurrence in RNA-binding proteins. Enhanced RGG citrullination has been reported in cells treated with A23187, a calcium ionophore. Citrullination by PAD4 is shown to suppress protein aggregation and sequestration into stress granules and a significant inhibition of aggregation of FUS, EWS, and TAF15 (FET) proteins has also been reported. This polyclonal antibody specifically recognized citrullinated RGG motifs. It detects multiple citrullinated proteins in HEK293T cells transfected with wild-type PAD4, but not in cells transfected with mutant PAD4. (Ref.: Tanikawa, C., et al. (2018). Cell Reports 22(6); 1473-1483; Kurowska, W., et al. (2017). Cent. Eur. J. Immunol. 42(4); 390-398).

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.