Anti-Adiponectin Antibody, Alexa Fluor^™ 488 Conjugate

Adiponectin (UniProt Q8K3R4; also known as 30 kDa adipocyte complement-related protein, ACRP30, Adipocyte complement-related 30 kDa protein, Adipocyte, C1q and collagen domain-containing protein, Adipose most abundant gene transcript 1 protein, apM-1, Gelatin-binding protein) is encoded by the Adipoq (also known as Acdc, Acrp30) gene (Gene ID 246253) in rat. Adipokines are adipose tissue-secreted factors involved in vascular function and metabolic disease. While most adipokines are proinflammatory and directly responsible for the development of the chronic inflammatory state in obesity, adiponectin is an adipokine with anti-inflammatory and insulin-sensitizing properties. Adiponectin mediates it biological activity via adiponection receptor protein 1 & 2 (AdipoR1 & AdipoR2). Both AdipoR1 and AdipoR2 are reported to be ubiquitously expressed with the highest expression in skeletal muscle and liver, respectively. Circulating adiponectin levels are reduced in obesity and type II diabetes, whereas weight loss elevates serum adiponectin. Likewise, adiponectin overexpression is reported to alleviate metabolic dysfunction independent of weight loss in both genetic and diet-induced animal models of obesity. Aadiponectin deficiency is associated with various clinical cardiovascular disorders including myocardial infarction, peripheral artery disease, and endothelial dysfunction. Adiponectin is produced with a signal peptide sequence (a.a. 1-18 in human and rat sequence; a.a. 1-17 in mouse), which is removed postranslationally to yield the mature protein with a collagen-like domain (a.a. 42-107 in human and rat, a.a. 45-110 in mouse) and a C1q domain (a.a. 108-244 in human and rat, a.a. 111-247 in mouse).

~33 kDa oberved. 24.92/26.81 kDa (mouse mature/pro-form) and 26.41/24.41 kDa (rat mature/pro-form) calculated.

GST-tagged recombinant rat adiponectin collagen-like domain.

Detect Adiponectin using this rabbit polyclonal Anti-Adiponectin Antibody, Alexa Fluor^™ 488 Conjugate, Cat. No. ABS516-AF488, validated for use in Immunofluorescence staining.

Immunofluorescence Analysis: A 1:150 dilution from a representative lot detected adiponectin in rat kidney tissue section.

The unconjugated antibody (Cat. No. ABS516) is also available for immunohistochemistry and Western blotting applications.

Research Category
Signaling

This Alexa Fluor^™ 488 conjugate was prepared from purified rabbit polyclonal antibody (Cat. No. ABS516) that was raised against rat adiponectin collagen-like domain and validated for target band detection in both rat and mouse serum samples by Western blotting. Human and Rat adiponectins share 82% sequence identity in the collagen-like domain.

Affinity purified.

Purified rabbit polyclonal antibody conjugate in PBS with 1.5% BSA and 0.05% sodium azide.

Stable for 1 year at 2-8°C from date of receipt.

Evaluated by Immunofluorescence in rat heart tissue.

Immunofluorescence Analysis: A 1:150 dilution of this antibody detected adiponectin in rat heart tissue section.

Concentration: Please refer to lot specific datasheet.

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