Anti-Perilipin-4, N-term Antibody

Perilipin-4, also known as PLIN4, or Adipocyte protein S3-12, and encoded by the gene PLIN4/KIAA1881, plays a role in triacylglycerol metabolism in adipocytes and governs the biogenesis of lipid droplets. Perilipin-4 is also necessary for proper adipocyte maturation. Because of its role in lipid droplet formation and their involvement in the formation of macrophagic foam cells, Perilipin 4 and its other family members are seen as key players in atherogenesis : The development of atherosclerotic plaques in the blood vessels. Thus Perilipin-4 and its family members are a focus of much research from metabolic syndrome to vascular disease. Perilipin-4 is a membrane protein found in adipocyte precursors as well as mature fat cells. Interestingly too, certain polymorphims in PLIN4 are associated with height in both males and females and suggests that PLIN4 and its other family members may be involved in general growth and development as well as adipogenesis.

~ 160 kDa observed. Uncharacterized band(s) may appear in some lysates.

Epitope: N-terminus

Linear peptide corresponding to the N-terminus of mouse Perilipin-4.

Immunohistochemistry Analysis: A representative lot detected Perilipin-4 in mouse fat tissue (Wolins, N. E., et al. (2003). JBC. 278(39):37713-37721).
Immunocytochemistry Analysis: A representative lot detected Perilipin-4 in 3T3_L1 cells (Wolins, N. E., et al. (2003). JBC. 278(39):37713-37721).
Western Blotting Analysis: A representative lot detected Perilipin-4 in White Adipose Tissue (WAT) (Wolins, N. E., et al. (2003). JBC. 278(39):37713-37721).

Research Category
Signaling

Research Sub Category
Signaling Neuroscience

This Anti-Perilipin-4 antibody, N-term is validated for use in western blotting, IHC & ICC for the detection of Perilipin-4.

Affinity purified

Purified rabbit polyclonal in buffer containing PBS without preservatives with 10% glycerol.

Stable for 1 year at -20°C from date of receipt.
Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
Note: Variability in freezer temperatures below -20°C may cause glycerol containing solutions to become frozen during storage.

Evaluated by Western Blotting in NIH/3T3-L1 cell lysate.

Western Blotting Analysis: A 1:1,000 of this antibody detected Perilipin-4 in 10 µg of NIH/3T3-L1 cell lysate.

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.