Syndecan-4 (Amphiglycan; Ryudocan core protein) is one of four members of the syndecan proteoglycan family. The syndecan proteins bind heparan sulfate and mediate the signaling of growth factors, such as fibroblast growth factor, that recognize and bind heparan chains. Unlike other members of this family, syndecan-4 is ubiquitously expressed, and is targeted to focal adhesions, possibly by a PKC-mediated pathway. Previous studies have reported an increase in focal adhesions in cells overexpressing syndecan-4. This protein also plays an important role in wound healing, by modulating the signaling of Rho-GTPases Rac1, RhoG, and RhoA.

~80/32 kDa observed

Epitope: Cytoplasmic domain

KLH-conjugated linear peptide corresponding to the cytoplasmic domain of human Syndecan-4.

Immunohistochemistry Analysis: A 1:100-1,000 dilution from a representative lot detected Syndecan-4 in human lung carcinoma and in distal tubules of normal human kidney tissue.

Research Category
Cell Structure

Research Sub Category
Cytoskeletal Signaling

This Anti-Syndecan-4 Antibody is validated for use in Western Blotting, IHC(P) for the detection of Syndecan-4.

This antibody recognizes the cytoplasmic domain of Syndecan-4

Affinity purified

Purified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Stable for 1 year at 2-8°C from date of receipt.

Control
HEP2 cell lysate

Evaluated by Western Blot in HEP2 cell lysate.

Western Blot Analysis: 1 µg/mL of this antibody detected Syndecan-4 in 10 µg of HEP2 cell lysate.

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.