Anti-Neomycin Phosphotransferase II Antibody | AC113

Neomycin phosphotransferase II confers resistance to kanamycin and neomycin in bacteria and G418 (Geneticin^®, G418 Sulfate) in mammalian cells. These antibiotics bind to ribosomal components and inhibit protein synthesis. NPT II inhibits these antibiotics through phosphorylation, which is thought to interfere with their active transport into the cell. Immunoblot analysis of transfected cell lysates with anti NPT II can be used to gauge relative transfection efficiencies in experiments where multiple transfections are performed in parallel with different plasmids.

~29 kDa observed.

Recombinant protein corresponding to full length neomycin phosphotransferase (NPT) II.

Research Category
Secondary & Control Antibodies

Research Sub Category
Kinases & Phosphatases

This antibody reacts to lysates from cells transfected or transformed with a plasmid containing a kanamycin/neomycin resistance gene, nptII (Aminoglycoside 3′-phosphotransferase).

Affinity purified

Hek 293 +/- transfection with pUSEamp(-)and neomycin treatment.

Purified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Stable for 1 year at 2-8°C from date of receipt.

Control
Untreated and neomycin treated HEK293 cells transfected with pUSEamp(-).

Evaluated by Western Blot in untreated and neomycin treated HEK293 cells transfected with pUSEamp(-).

Western Blot Analysis: A 1:1,000 dilution of this antibody detected Neomycin phosphotransferase II in 10 µg of neomycin treated HEK293 cells transfected with pUSEamp(-).

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