ACS505002
Amicon® Pro Purification System with Ultra-0.5 Device
Amicon Pro Purification System - A 50 kDa Centrifugal Tool collapsing protein purifcation with concentraion & buffer exchange.
material
Amicon Ultra styrene-butadiene device, Ultracel® regenerated cellulose membrane (Amicon Ultra-0.5; low binding), polyethylene cap, polyethylene frit, polypropylene device (for exchange), polypropylene frit, polypropylene tube (AU0.5; for collection), polypropylene tube (for collection), polypropylene tube (holder)
sterility
non-sterile
product line
Amicon®
manufacturer/tradename
Amicon® Pro
parameter
10 mL sample volume
technique(s)
buffer exchange: suitable, protein purification: suitable (antibody labeling), protein purification: suitable (concentration), protein purification: suitable (enrichment)
L
12 cm (4.7 in.)
diam.
2.97 cm
filtration area
1 cm2
pore size
50 kDa NMWCO
shipped in
ambient
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General description
Application
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相关内容
bios-C-epdf-1043-1304-SP
Two attributes of the Amicon® Pro device make it a convenient device for preparing pure, labeled antibody. First, the device enables highly efficient buffer exchange via diafiltration with simultaneous sample concentration in a single 15 minute spin. Second, the entire workflow can be performed within a single device, reducing the potential for sample loss. In this report, we describe the successful use of the Amicon® Pro device for small–scale biotinylation of a target antibody. The biotinylated antibody was used for detection of protein immunoprecipitated from cell lysate.
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