生物来源
mouse
质量水平
抗体形式
purified antibody
抗体产品类型
primary antibodies
克隆
EMD-17D7, monoclonal
表单
liquid
包含
≤0.1% sodium azide as preservative
种属反应性
human
制造商/商品名称
Calbiochem®
储存条件
OK to freeze
avoid repeated freeze/thaw cycles
dilution
(ELISA
Immunoblotting (1 µg/mL)
Immunoprecipitation (5 µg/0.7 mg protein))
同位素/亚型
IgG1
运输
wet ice
储存温度
−20°C
靶向翻译后修饰
unmodified
一般描述
Protein G PLUS/protein A purified mouse monoclonal antibody. Recognizes the ~90 kDa Hsp90α protein.
Recognizes the ~90 kDa Hsp90α protein in HeLa cells. Does not recognize Hsp90β.
This Anti-Hsp90α Mouse mAb (EMD-17D7) is validated for use in ELISA, Immunoblotting, Immunoprecipitation for the detection of Hsp90α.
免疫原
Hsp90α, His•Tag, Human, Recombinant (Cat. No. 385901)
Human
应用
ELISA (see comments)
Immunoblotting (1 g/ml)
Immunoprecipitation (5 g/0.7 mg protein)
包装
Please refer to vial label for lot-specific concentration.
外形
In PBS.
制备说明
Following initial thaw, aliquot and freeze (-20°C).
分析说明
Positive Control
H460 and HeLa cells
H460 and HeLa cells
其他说明
Detects native and recombinant Hsp90α. Does not recognize Hsp90β. This antibody will also work for ELISA, but concentration is assay dependent. Antibody should be titrated for optimal results in individual systems.
Scheibel, T., et al. 1999. Proc. Natl. Acad. Sci. USA96, 1297.
Nemoto, T., et al. 1997 J. Biol. Chem.272, 26179.
Nemoto, T., et al. 1998. Biochem. J.330, 989.
Nemoto, T., et al. 1997 J. Biol. Chem.272, 26179.
Nemoto, T., et al. 1998. Biochem. J.330, 989.
法律信息
CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany
免责声明
Toxicity: Irritant (B)
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储存分类代码
10 - Combustible liquids
WGK
WGK 1
闪点(°F)
Not applicable
闪点(°C)
Not applicable
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