biological source
mouse
Quality Level
antibody form
purified antibody
antibody product type
primary antibodies
clone
4H8, monoclonal
form
liquid
does not contain
preservative
species reactivity
human, rat
manufacturer/tradename
Calbiochem®
storage condition
OK to freeze, avoid repeated freeze/thaw cycles
isotype
IgG2a
shipped in
wet ice
storage temp.
−20°C
target post-translational modification
unmodified
Gene Information
rat ... Mfn2(64476)
General description
Purified mouse monoclonal antibody. Recognizes the ~75 kDa MFN2 protein.
Recognizes the ~75 kDa MFN2 protein in HeLa and PC-12 cells and human heart tissue.
This Anti-MFN2 Mouse mAb (4H8) is validated for use in ELISA, Immunoblotting, Paraffin Sections for the detection of MFN2.
Immunogen
A recombinant polypeptide corresponding to amino acids 661-758 of human MFN2, expressed as a GST fusion protein
NA
Application
ELISA (see comments)
Immunoblotting (1-5 g/ml)
Paraffin Sections (5 g/ml)
Physical form
In PBS, pH 7.2.
Preparation Note
Following initial thaw, aliquot and freeze (-20°C).
Analysis Note
Positive Control
HeLa cells, PC-12 cells, Human heart tissue
HeLa cells, PC-12 cells, Human heart tissue
Other Notes
For ELISA, this antibody can be used as the capture antibody; recommended concentration is 10 ng/ml, 1 µg/well, 100 µl total volume. Variables associated with assay conditions will dictate the proper working dilution.
Moran, M., et al. 2010. Biochim Biophys. Acta.1802 443.
Holloway, G,P., et al. 2008. Am. J. Physiol. Endocrinol. Metab.294 E1060.
Holloway, G,P., et al. 2008. Am. J. Physiol. Endocrinol. Metab.294 E1060.
Legal Information
CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
Toxicity: Regulatory Review (Z)
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存储类别
12 - Non Combustible Liquids
wgk
nwg
flash_point_f
Not applicable
flash_point_c
Not applicable
null
全球贸易项目编号
| 货号 | GTIN |
|---|---|
| CB1020-100UG | 04055977228670 |