一般描述
This product has been discontinued.
The K-LISA Akt Activity Kit is a rapid, sensitive, ELISA-based activity assay suitable for measuring the kinase activity of purified or partially purified Akt preparations, in vitro Akt inhibitor screening, and for assessing the regulation of Akt in cell signaling. The assay utilizes a biotinylated peptide substrate (GRPRTSSFAEG) that is phosphorylated on the second serine by Akt1, Akt2, Akt3, SGK (Serum Glucocorticoid Kinase), and MSK1. Biotinylated Akt substrate and sample containing Akt are incubated in the presence of ATP in wells of a streptavidin-coated 96-well plate, which allows for phosphorylation and substrate capture in a single step.
组分
Biotinylated Akt substrate, phosphoserine detector antibody, streptavidin coated 96-well plate strips, HRP-conjugated secondary antobody, serine/threonine kinase assay buffer, ATP/MgCl₂, inhibitor, wash buffer, TMB substrate, stop solution, EDTA solution, plate sealers, and a user protocol. Purified Akt is available separately (Cat. No. 124006).
Streptavidin-Coated 96-Well Plate, Akt Substrate, Biotinylated, Phosphoserine Detection Antibody, Antibody Diluent, Kinase Assay Buffer, 5X ATP/MgCl₂ Mix, 100X Inhibitor, Kinase Stop Solution, ELISA 20X Plate Wash Concentrate, HRP-Antibody Conjugate, TMB Substrate, ELISA Stop Solution, Plate Sealers, and a user protocol. Sold under license of U.S. patent 6,441,140. Manufactured by Cell Signaling.
警告
Toxicity: Multiple Toxicity Values, refer to MSDS (O)
原理
The K-LISA Akt Activity Kit is suitable for measuring the kinase activity of purified or partially purified Akt preparations, in vitro Akt inhibitor screening, and for assessing the regulation of Akt in cell signaling.
单位定义
One unit is defined to the amount of enzyme required to transfer 1 nmol phosphate per min to substrate at 30° C.
制备说明
Prepare all reagents immediately prior to use. The following table provides reagent preparation instructions to obtain the
Volume of Working Solutions (WS) required for
one 8-well strip:
Table 1: Preparation of Reagants
储存及稳定性
Upon arrival store the Streptavidin-Coated 96-Well Plate at 4°C and the remaining components of the kit at -20°C.
其他说明
Due to the nature of the Hazardous Materials in this shipment, additional shipping charges may be applied to your order. Certain sizes may be exempt from the additional hazardous materials shipping charges. Please contact your local sales office for more information regarding these charges.
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法律信息
*Sold under license of U.S. patent 6,441,140Manufactured by Cell Signaling
CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany
